Abstract：
For centuries plants have been intensively utilized as reliable sources of food, flavoring, and pharmaceutical ingredients. However, plant natural habitats are being rapidly lost due to the climate change and agriculture. Plant biotechnology offers a sustainable approach for the bioproduction of specialized plant metabolites. The unique structural features of plant-derived specialized metabolites, such as their safety profile and multi-target spectrum, have led to the establishment of many plant-derived drugs. However, there are still many challenges to overcome regarding the production of these metabolites from plant in vitro systems and establish a sustainable large-scale biotechnological process. These challenges are due to the peculiarities of plant cell metabolism, the complexity of plant specialized metabolite pathways, and the correct selection of bioreactor systems and bioprocess optimization. In this book chapter, we attempted to focus on the advantages of plant in vitro systems and in particular plant cell suspensions for their cultivation as a source of plant-derived specialized metabolites. A state-of-the-art technological platform for plant cell suspension cultivation from callus induction to lab-scale cultivation, extraction, and purification is presented. Possibilities for bioreactor cultivation of plant cell suspensions in benchtop and large-scale volumes are highlighted, including several examples and patents for industrial production of specialized metabolites.
摘要：
几个世纪以来，植物一直被广泛用作可靠的食物来源，调味,和药物成分。然而,由于气候变化和农业，植物的自然栖息地正在迅速丧失。植物生物技术为专门的植物代谢物的生物生产提供了可持续的方法。植物衍生的特殊代谢产物的独特结构特征，比如他们的安全特征和多目标光谱，导致了许多植物衍生药物的建立。然而,从植物体外系统生产这些代谢物和建立可持续的大规模生物技术过程仍有许多挑战需要克服。这些挑战是由于植物细胞代谢的特殊性，植物特殊代谢途径的复杂性，以及生物反应器系统的正确选择和生物工艺优化。在这本书的章节中，我们试图关注植物体外系统的优势，特别是植物细胞悬浮液作为植物衍生的专门代谢产物的来源。从愈伤组织诱导到实验室规模培养的植物细胞悬浮培养的最先进的技术平台，提取,并提出了纯化。强调了在台式和大规模体积中生物反应器培养植物细胞悬浮液的可能性，包括几个用于工业生产专门代谢物的例子和专利。
