Abstract：
Somatic embryogenesis (SE) is a clear example of cellular totipotency. The SE of the genus Coffea has become a model for in vitro propagation for woody species and for the large-scale production of disease-free plants that provide an advantage for modern agriculture. Temporary immersion systems (TIS) are in high demand for the propagation of plants. The success of this type of bioreactor is based on the alternating cycles of immersion of the plant material in the culture medium, usually a few minutes, and the permanence outside the medium of the tissues for several hours. Some bioreactors are very efficient for propagating one species but not another. The efficiency of bioreactors depends on the species, the tissue used to propagate, the species\' nutritional needs, the amount of ethylene produced by the tissue, and many more. In this protocol, we show how we produce C. canephora plants that are being taken to the field.
摘要：
体细胞胚发生(SE)是细胞全能性的明显例子。咖啡属的SE已成为木本物种体外繁殖和大规模生产无病植物的模型，为现代农业提供了优势。临时浸没系统（TIS）对植物繁殖的需求很高。这种生物反应器的成功是基于植物材料在培养基中的交替浸入循环。通常是几分钟,以及在组织培养基外几个小时的持久性。一些生物反应器对于繁殖一种物种而不是另一种物种非常有效。生物反应器的效率取决于物种，用来繁殖的组织，物种的营养需求，组织产生的乙烯量，还有更多。在这个协议中,我们展示了我们如何生产被带到田间的C.canephora植物。
