Abstract：
Plant cell suspension cultures (PCSCs) are in vitro-cultured cells that can divide indefinitely in a sterile growth medium. These PCSCs can be derived from various plant tissues, such as the root, stem, leaves, or seeds, and are maintained in a suitable culture medium containing nutrients, vitamins, hormones, and other essential components necessary for their growth. PCSCs have extensive applications in biotechnology, particularly in producing pharmaceutical and chemical compounds. This chapter presents a protocol for generating cell lines from Arabidopsis thaliana root callus under different light conditions, which can be used to investigate the effects of light on plant cell growth and development. The protocol described in this chapter is a valuable tool for researchers interested in utilizing PCSCs in their studies.
摘要：
植物细胞悬浮培养物（PCSC）是体外培养的细胞，可以在无菌生长培养基中无限分裂。这些PCSCs可以来源于各种植物组织，比如根，茎,叶子,或种子，并保持在含有营养物质的合适培养基中，维生素,荷尔蒙，以及他们成长所必需的其他重要组成部分。PCSCs在生物技术中有着广泛的应用,特别是在生产药物和化学化合物中。本章介绍了在不同光照条件下从拟南芥根愈伤组织产生细胞系的方案，可用于研究光照对植物细胞生长发育的影响。本章中描述的协议对于有兴趣在研究中利用PCSCs的研究人员来说是一个有价值的工具。
