Abstract：
Introduction. Maternal screening tests and prophylactic antibiotics are important to prevent neonatal and infant group B streptococcal (GBS) infections.Hypothesis/Gap Statement. The performance of enrichment broth media for GBS screening that are available in Japan is unclear. Whole-genome data of GBS isolates from pregnant women in Japan is lacking.Aim. The aim of this study was to compare the protocol performance of six enrichment broths and two subculture agar plates, which were all available in Japan, for GBS detection. In addition, we showed whole-genome data of GBS isolates from pregnant women in Japan.Methodology. We collected 133 vaginal-rectal swabs from pregnant women visiting clinics and hospitals in Nagasaki Prefecture, Japan, and compared the protocol performance of 6 enrichment broths and 2 subculture agar plates. All GBS isolates collected in this study were subjected to whole-genome sequencing analysis.Results. We obtained 133 vaginal-rectal swabs from pregnant women at 35-37 weeks of gestation from 8 private clinics and 2 local municipal hospitals within Nagasaki Prefecture, Japan. The detection rate of the protocol involving the six enrichment broths and subsequent subcultures varied between 95.5 and 100 %, depending on the specific choice of enrichment broth. The GBS carriage rate among pregnant women in this region was 18.8 %. All 25 isolates derived from the swabs were susceptible to penicillin, whereas 48 and 36 % of the isolates demonstrated resistance to erythromycin and clindamycin, respectively. The distribution of serotypes was highly diverse, encompassing seven distinct serotypes among the isolates, with the predominant serotype being serotype V (n = 8). Serotype V isolates displayed a tendency towards increased resistance to erythromycin and clindamycin, with all resistant isolates containing the ermB gene.Conclusion. There was no difference in performance among the culture protocols evaluated in this study. GBS strains isolated from pregnant women appeared to have greater genomic diversity than GBS strains detected in neonates/infants with invasive GBS infections. To confirm this result, further studies with larger sample sizes are needed.
摘要：
Introduction.孕产妇筛查试验和预防性抗生素对预防新生儿和婴儿B组链球菌（GBS）感染很重要。假设/差距声明。在日本可获得的用于GBS筛选的富集肉汤培养基的性能尚不清楚。缺乏日本孕妇GBS分离株的全基因组数据。瞄准.这项研究的目的是比较六个富集肉汤和两个继代培养琼脂平板的方案性能，在日本都有，用于GBS检测。此外,我们展示了日本孕妇GBS分离株的全基因组数据.方法论。我们从长崎县诊所和医院的孕妇那里收集了133份阴道直肠拭子，Japan,并比较了6个富集培养液和2个继代培养琼脂平板的方案性能。对在本研究中收集的所有GBS分离物进行全基因组测序分析。结果。我们从长崎县的8家私人诊所和2家当地市政医院获得了妊娠35-37周孕妇的133份阴道直肠拭子，日本。涉及六个富集肉汤和随后的继代培养的方案的检出率在95.5％和100％之间变化。根据具体选择的富集肉汤。该地区孕妇的GBS携带率为18.8%。来自拭子的所有25个分离株都对青霉素敏感，而48％和36％的分离株表现出对红霉素和克林霉素的抗性，分别。血清型分布高度多样化，在分离株中包含7种不同的血清型，主要的血清型是血清型V(n=8)。血清型V分离株对红霉素和克林霉素的耐药性有增加的趋势，所有抗性分离株都含有ermB基因。结论。在这项研究中评估的培养方案之间的性能没有差异。从孕妇中分离的GBS菌株似乎比在具有侵袭性GBS感染的新生儿/婴儿中检测到的GBS菌株具有更大的基因组多样性。为了确认这个结果，需要更大样本量的进一步研究。
