Abstract：
Bisphenol A (BPA) may adversely affect human health by inducing oxidative stress and irreversible damage to cells. Bioactive compounds found in some functional foods, individually or in combination, can attenuate the negative effects of BPA exposure; an example is the multi-supplement containing guarana (Gua), selenium (Se), and L-carnitine (LC) -GSC- which has already demonstrated antioxidant, genoprotective, and immunomodulatory activities. This study aimed to determine the effect of GSC and its constituents on oxidative and genotoxic alterations triggered by BPA exposure in the retinal epithelial cell line. The cells exposed to BPA (0.001, 0.01, 0.1, 1, 3, and 10 µM) to determine the lowest concentration required to induce cyto-genotoxicity. ARPE-19 cells were then concomitantly exposed to the selected BPA concentration, GSC, and its components (Gua, 1.07 mg/mL; Se, 0.178 µg/mL; and LC, 1.43 mg/mL). Flow cytometry, biochemical assays, qRT-PCR, genotoxicity, apoptosis, and cellular proliferation. Based on our results, 10 µM of BPA could induce cyto-genotoxic and oxidative alterations. BPA did not alter the Bcl-2/BAX expression ratio but induced Casp3 and Casp8 overexpression, suggesting that apoptosis was induced mainly via the extrinsic pathway. GSC partially reversed the alterations triggered by BPA in ARPE-19 cells. However, Se had unexpected negative effects on ARPE-19 cells. The multi-supplement GSC may attenuate changes in oxidative and genotoxic markers related to exposure of ARPE-19 cells to BPA. our results revealed that the antioxidant, anti-apoptotic, and genoprotective properties of GSC were not universally shared by its individual, once Se did not exhibit any positive impact.
摘要：
双酚A（BPA）可能通过诱导氧化应激和对细胞的不可逆损伤而对人类健康产生不利影响。在一些功能性食品中发现的生物活性化合物，单独或组合，可以减弱双酚A暴露的负面影响；一个例子是含有瓜拉那（Gua）的多重补充剂，硒(Se),和L-肉碱（LC）-GSC-已经证明了抗氧化剂，基因保护,和免疫调节活动。这项研究旨在确定GSC及其成分对视网膜上皮细胞系中BPA暴露引发的氧化和遗传毒性改变的影响。将细胞暴露于BPA（0.001、0.01、0.1、1、3和10µM）以确定诱导细胞遗传毒性所需的最低浓度。然后将ARPE-19细胞同时暴露于选定的BPA浓度，GSC,及其组件(Gua，1.07mg/mL;Se,0.178微克/毫升；和LC，1.43mg/mL)。流式细胞术,生化化验,qRT-PCR,遗传毒性，凋亡，和细胞增殖。根据我们的结果,10µM的BPA可以诱导细胞基因毒性和氧化性改变。BPA不改变Bcl-2/BAX表达比例，但诱导Casp3和Casp8过表达，提示细胞凋亡主要通过外源性途径诱导。GSC部分逆转了ARPE-19细胞中BPA引发的改变。然而,硒对ARPE-19细胞具有意想不到的负面影响。多补充性GSC可以减弱与ARPE-19细胞暴露于BPA相关的氧化和基因毒性标志物的变化。我们的结果表明，抗氧化剂，抗凋亡，GSC的基因保护特性并没有被其个人普遍分享，一旦硒没有表现出任何积极的影响。
