Abstract：
The present study explored the potential of leaf litter as a source of fungi able to produce ligninolytic enzymes for the biodegradation of anthraquinone dyes. Within the colonies isolated from the leaf litter, only three colonies of two species Trametes were selected based on the detection of oxidation and decolorization halos in Petri dishes with PDA (potato-dextrose-agar) + Guaicol and PDA + RBBR (Remazol Brilliant Blue R). The identification of the colonies was done through sequencing of the ITS region. The enzymatic activity of Lac (lacase), MnP (manganês peroxidase) and LiP (lignina peroxidase) was analyzed by spectrophotometry during fermentation in PD+RBBR imedium. Isolates A1SSI01 and A1SSI02 were identified as Trametes flavida, while A5SS01 was identified as Trametes sp. Laccase showed the highest enzymatic activity, reaching 452.13 IU.L-1 (A1SSI01, 0.05% RBBR) after 96h. Isolate A1SSI02 reached the highest percentage of decolorization, achieving 89.28% in seven days. The results imply that these Trametes isolates can be highly effective in waste treatment systems containing toxic anthraquinone dyes. Keywords: laccase, peroxidases, basidiomycete, litter and biodecolorization.
摘要：
本研究探索了落叶作为真菌来源的潜力，该真菌能够产生木质素分解酶以生物降解蒽醌染料。在从落叶中分离出来的菌落内，根据在PDA（马铃薯-葡萄糖-琼脂）和PDARBBR（RemazolBrilliantBlueR）的培养皿中对氧化和脱色晕的检测，仅选择了两种Trametes的三个菌落。菌落的鉴定通过ITS区域的测序来完成。Lac（乳酶）的酶活性，在PDRBBRimedium中的发酵过程中，通过分光光度法分析了MnP（锰过氧化物酶）和LiP（木质素过氧化物酶）。分离株A1SSI01和A1SSI02被鉴定为黄藻，而A5SS01被鉴定为Trametessp。漆酶表现出最高的酶活性，达到452.13IU。L-1(A1SSI01,0.05%RBBR)后96h。分离物A1SSI02的脱色率最高，七天内实现89.28%。结果表明，这些Trametes分离物在含有有毒蒽醌染料的废物处理系统中可以非常有效。关键词:漆酶,过氧化物酶,担子菌,凋落物和生物脱色。
