Abstract：
OBJECTIVE: Our research focused on plant\'s ethanolic extract Lavandula stoechas flower part to investigate the potential analgesic effects and possible pathways involvements.
METHODS: Four experimental tests were performed on Swiss albino mice with five animals in each group at different doses (50, 100, and 200mg/kg); formalin test, tail-flick test, acetic acid-induced writhing, and hot-plate test. The opioidergic, noradrenergic, cholinergic, and K channel blockers in the analgesic actions were also carried out for the potential route involvement.
RESULTS: The percentage inhibition for abdominal writhing\'s and formalin activity showed a dose-dependent manner for early and late phases reducing abdominal writhing\'s and time period of licking, respectively. Tail immersion and hot-plate test demonstrated a substantial and dose-dependent increase in the latency time and time period of paw liking and jumping response respectively. GC-MS showed the abundantly present compounds were octadecatrienoic acid (34.35%), n-hexadecanoic acid (12.98%). In silico analyses have revealed three compounds that had good interactions with 6y3c receptor proteins, demonstrating strong binding affinities and satisfying docking parameters.
CONCLUSIONS: Overall, these studies showed that ethanolic extract of L. stoechas is an important medicinal plant, with both central and peripheral antinociceptive and analgesic activities supporting its traditional use for therapeutic purposes.
METHODS: Four experimental tests were performed on Swiss albino mice with five animals in each group at different doses (50, 100, and 200mg/kg); formalin test, tail-flick test, acetic acid-induced writhing, and hot-plate test. The opioidergic, noradrenergic, cholinergic, and K channel blockers in the analgesic actions were also carried out for the potential route involvement.
RESULTS: The percentage inhibition for abdominal writhing\'s and formalin activity showed a dose-dependent manner for early and late phases reducing abdominal writhing\'s and time period of licking, respectively. Tail immersion and hot-plate test demonstrated a substantial and dose-dependent increase in the latency time and time period of paw liking and jumping response respectively. GC-MS showed the abundantly present compounds were octadecatrienoic acid (34.35%), n-hexadecanoic acid (12.98%). In silico analyses have revealed three compounds that had good interactions with 6y3c receptor proteins, demonstrating strong binding affinities and satisfying docking parameters.
CONCLUSIONS: Overall, these studies showed that ethanolic extract of L. stoechas is an important medicinal plant, with both central and peripheral antinociceptive and analgesic activities supporting its traditional use for therapeutic purposes.
摘要：
目的：我们的研究集中在植物的乙醇提取物Lavandulastoechas花部分，以研究潜在的镇痛作用和可能的途径。
方法：对瑞士白化病小鼠进行了四个实验测试，每组五只动物以不同的剂量（50、100和200mg/kg）；福尔马林试验，甩尾试验,乙酸引起的扭动，和热板测试。opiopideric,去甲肾上腺素能,胆碱能，和K通道阻滞剂在镇痛作用中也进行了潜在的途径参与。
结果：腹部扭体和福尔马林活性的抑制百分比显示出早期和晚期减少腹部扭体和舔时期的剂量依赖性方式，分别。尾部浸入和热板测试表明，爪子喜欢和跳跃反应的潜伏期和时间段分别显着增加和剂量依赖性。GC-MS显示大量存在的化合物是十八碳三烯酸（34.35％），正十六烷酸(12.98%)。计算机分析显示，三种化合物与6y3c受体蛋白具有良好的相互作用，表现出很强的结合亲和力和令人满意的对接参数。
结论：总体而言，这些研究表明，L.stoecas的乙醇提取物是一种重要的药用植物，具有中枢和外周镇痛和镇痛活性，支持其用于治疗目的的传统用途。
方法：对瑞士白化病小鼠进行了四个实验测试，每组五只动物以不同的剂量（50、100和200mg/kg）；福尔马林试验，甩尾试验,乙酸引起的扭动，和热板测试。opiopideric,去甲肾上腺素能,胆碱能，和K通道阻滞剂在镇痛作用中也进行了潜在的途径参与。
结果：腹部扭体和福尔马林活性的抑制百分比显示出早期和晚期减少腹部扭体和舔时期的剂量依赖性方式，分别。尾部浸入和热板测试表明，爪子喜欢和跳跃反应的潜伏期和时间段分别显着增加和剂量依赖性。GC-MS显示大量存在的化合物是十八碳三烯酸（34.35％），正十六烷酸(12.98%)。计算机分析显示，三种化合物与6y3c受体蛋白具有良好的相互作用，表现出很强的结合亲和力和令人满意的对接参数。
结论：总体而言，这些研究表明，L.stoecas的乙醇提取物是一种重要的药用植物，具有中枢和外周镇痛和镇痛活性，支持其用于治疗目的的传统用途。
