PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to investigate the expression levels of progranulin (PGRN) in the tears of patients with diabetic retinopathy (DR) versus healthy controls. Additionally, we sought to explore the correlation between PGRN levels and the severity of ocular surface complications in patients with diabetes.
UNASSIGNED: In this prospective, single-visit, cross-sectional study, patients with DR (n = 48) and age-matched healthy controls (n = 22) were included and underwent dry eye examinations. Tear fluid was collected, and its components were analyzed using the Luminex assay. The subbasal nerve plexus of all participants was evaluated by in vivo confocal microscopy.
UNASSIGNED: Patients with DR exhibited more severe dry eye symptoms, along with a reduction in nerve fiber density, length, and branch density within the subbasal nerve plexus, accompanied by an increase in the number of dendritic cells. Tear PGRN levels were also significantly lower in patients with diabetes than in normal controls, and the levels of some inflammatory factors (TNF-α, IL-6, and MMP-9) were higher in patients with DR. Remarkably, the PGRN level significantly correlated with nerve fiber density (R = 0.48, P < 0.001), nerve fiber length (R = 0.65, P < 0.001), and nerve branch density (R = 0.69, P < 0.001).
UNASSIGNED: Tear PGRN levels might reflect morphological changes in the corneal nerve plexus under diabetic conditions, suggesting that PGRN itself is a reliable indicator for predicting the advancement of neurotrophic keratopathy in patients with diabetes.
UNASSIGNED: PGRN insufficiency on the ocular surface under diabetic conditions was found to be closely associated with nerve impairment, providing a novel perspective to discover the pathogenesis of diabetic complications, which could help in developing innovative therapeutic strategies.
UNASSIGNED: In this prospective, single-visit, cross-sectional study, patients with DR (n = 48) and age-matched healthy controls (n = 22) were included and underwent dry eye examinations. Tear fluid was collected, and its components were analyzed using the Luminex assay. The subbasal nerve plexus of all participants was evaluated by in vivo confocal microscopy.
UNASSIGNED: Patients with DR exhibited more severe dry eye symptoms, along with a reduction in nerve fiber density, length, and branch density within the subbasal nerve plexus, accompanied by an increase in the number of dendritic cells. Tear PGRN levels were also significantly lower in patients with diabetes than in normal controls, and the levels of some inflammatory factors (TNF-α, IL-6, and MMP-9) were higher in patients with DR. Remarkably, the PGRN level significantly correlated with nerve fiber density (R = 0.48, P < 0.001), nerve fiber length (R = 0.65, P < 0.001), and nerve branch density (R = 0.69, P < 0.001).
UNASSIGNED: Tear PGRN levels might reflect morphological changes in the corneal nerve plexus under diabetic conditions, suggesting that PGRN itself is a reliable indicator for predicting the advancement of neurotrophic keratopathy in patients with diabetes.
UNASSIGNED: PGRN insufficiency on the ocular surface under diabetic conditions was found to be closely associated with nerve impairment, providing a novel perspective to discover the pathogenesis of diabetic complications, which could help in developing innovative therapeutic strategies.
摘要：
■本研究旨在研究与健康对照相比，糖尿病性视网膜病变（DR）患者泪液中颗粒蛋白前体（PGRN）的表达水平。此外,我们试图探讨糖尿病患者PGRN水平与眼表并发症严重程度之间的相关性.
■在这个前景中，单次访问,横断面研究，纳入DR患者(n=48)和年龄匹配的健康对照者(n=22),并接受干眼检查.收集泪液,及其成分使用Luminex测定法进行分析。通过体内共聚焦显微镜评估所有参与者的基底下神经丛。
■DR患者表现出更严重的干眼症状，随着神经纤维密度的降低，长度,和基底下神经丛内的分支密度，伴随着树突状细胞数量的增加。糖尿病患者的泪液PGRN水平也明显低于正常对照组，和一些炎症因子(TNF-α，DR患者的IL-6和MMP-9）较高。值得注意的是,PGRN水平与神经纤维密度显著相关(R=0.48,P<0.001),神经纤维长度(R=0.65,P<0.001),神经分支密度(R=0.69,P<0.001)。
■泪液PGRN水平可能反映了糖尿病状态下角膜神经丛的形态学变化，提示PGRN本身是预测糖尿病患者神经营养性角膜病变进展的可靠指标。
■糖尿病患者眼表PGRN功能不全与神经损伤密切相关，为发现糖尿病并发症的发病机制提供了一个新的视角，这可以帮助开发创新的治疗策略。
■在这个前景中，单次访问,横断面研究，纳入DR患者(n=48)和年龄匹配的健康对照者(n=22),并接受干眼检查.收集泪液,及其成分使用Luminex测定法进行分析。通过体内共聚焦显微镜评估所有参与者的基底下神经丛。
■DR患者表现出更严重的干眼症状，随着神经纤维密度的降低，长度,和基底下神经丛内的分支密度，伴随着树突状细胞数量的增加。糖尿病患者的泪液PGRN水平也明显低于正常对照组，和一些炎症因子(TNF-α，DR患者的IL-6和MMP-9）较高。值得注意的是,PGRN水平与神经纤维密度显著相关(R=0.48,P<0.001),神经纤维长度(R=0.65,P<0.001),神经分支密度(R=0.69,P<0.001)。
■泪液PGRN水平可能反映了糖尿病状态下角膜神经丛的形态学变化，提示PGRN本身是预测糖尿病患者神经营养性角膜病变进展的可靠指标。
■糖尿病患者眼表PGRN功能不全与神经损伤密切相关，为发现糖尿病并发症的发病机制提供了一个新的视角，这可以帮助开发创新的治疗策略。
