PDF(Pubmed)
Abstract：
Regenerative therapy is considered a novel option for treating various diseases, whereas a developing embryo is a prime source of molecules that help repair diseased tissue and organs. Organoid culture studies also confirmed the inherent biological functions of several embryonic factors. However, the in vivo safety and efficacy of embryonic protein fraction (EPF) were not validated. In this study, we investigated the effectiveness of EPF on healthy adult rats. We obtained embryos from Sprague-Dawley (SD) female rats of E14, E16, and E19 embryonic days and collected protein lysate. This lysate was administered intravenously into adult SD rats on sequential days. We collected blood and performed hematological and biochemical parameters of rats that received EPF. C-reactive protein levels, interleukin-6, blood glucose levels, serum creatinine, blood urea, total leucocyte counts, and % of neutrophils and lymphocytes were comparable between rats receiving EPF and saline. Histological examination of rats\' tissues administered with EPF is devoid of abnormalities. Our study revealed that intravenous administration of EPF to healthy adult rats showed that EPF is non-immunogenic, non-inflammatory, non-tumorigenic, and safe for in vivo applications. Our analysis suggests that EPF or its components could be recommended for validating its therapeutic abilities in organ regenerative therapy.
摘要：
再生疗法被认为是治疗各种疾病的新选择。而发育中的胚胎是帮助修复患病组织和器官的主要分子来源。类器官培养研究还证实了几种胚胎因子的固有生物学功能。然而,胚胎蛋白组分(EPF)的体内安全性和有效性未得到验证.在这项研究中,我们研究了EPF对健康成年大鼠的有效性。我们从E14，E16和E19胚胎天的SD雌性大鼠中获得了胚胎，并收集了蛋白质裂解液。在连续几天将该裂解物静脉内给予成年Sprague-Dawley大鼠。我们收集血液并对接受EPF的大鼠进行血液学和生化指标。C反应蛋白水平,白细胞介素-6，血糖水平，血清肌酐,血尿素,白细胞总数,在接受EPF和生理盐水的大鼠之间，中性粒细胞和淋巴细胞的百分比相当。给予EPF的大鼠组织的组织学检查没有异常。我们的研究表明，向健康成年大鼠静脉注射EPF表明EPF是非免疫原性的，非炎性,非致瘤和安全的体内应用。我们的分析表明，EPF或其组件可以推荐用于验证其在器官再生治疗中的治疗能力。
