Abstract：
Ramelteon (RMLT) is a melatonin receptor agonist that it has antioxidative and anti-inflammatory effects associated with DNA damage through different mechanisms of action. In this regard, we investigated the potential usefulness of RMLT as a protective agent against methotrexate (MTX)-induced DNA damage. Four groups were constituted from 32 Wistar albino rats: Negative control, RMLT, MTX, and MTX + RMLT. Twenty mg/kg MTX (i.p., single dose) and RMLT 10 mg/kg (oral, 7 days) was administered. Comet assay was used and the parameter %TailDNA was used to detect DNA damage. %TailDNA was 4.90 ± 0.19 in the control group, 7.85 ± 0.33 in the MTX group, 5.49 ± 0.24 in the RMLT group, and 5.86 ± 0.23 in the MTX + RMLT group. While there was a significant increase in DNA damage in the MTX-treated group compared to the control group, there was a significant reduction in DNA damage in the MTX + RMLT group, compared to the MTX group (p < 0.001). In conclusion, it was observed that combined treatment with RMLT significantly reduced MTX-induced DNA damage.
Investigate the possible protective effect of RMLT against DNA damage caused by MTX using the comet method.The DNA damage of RMLT treated group was significantly reduced compared to group and MTX group. (p < 0.001).Combined treatment with MTX significantly reduces MTX-induced DNA damage.
Investigate the possible protective effect of RMLT against DNA damage caused by MTX using the comet method.The DNA damage of RMLT treated group was significantly reduced compared to group and MTX group. (p < 0.001).Combined treatment with MTX significantly reduces MTX-induced DNA damage.
摘要：
Ramelteon（RMLT）是一种褪黑激素受体激动剂，通过不同的作用机制具有与DNA损伤相关的抗氧化和抗炎作用。在这方面,我们研究了RMLT作为甲氨蝶呤(MTX)诱导的DNA损伤的保护剂的潜在用途.由32只Wistar白化病大鼠组成四组：阴性对照，RMLT,MTX,和MTX+RMLT。20mg/kgMTX(i.p.，单剂量）和RMLT10mg/kg（口服，7天）给药。使用彗星测定,并使用参数%TailDNA来检测DNA损伤。对照组的%TailDNA为4.90±0.19，MTX组7.85±0.33，RMLT组5.49±0.24，MTX+RMLT组5.86±0.23。虽然与对照组相比，MTX治疗组的DNA损伤显着增加，MTX+RMLT组DNA损伤显著减少，与MTX组相比（p<0.001）。总之,观察到与RMLT的联合治疗显着减少了MTX诱导的DNA损伤。
使用彗星方法研究RMLT对MTX引起的DNA损伤的可能保护作用。RMLT处理组的DNA损伤与MTX组相比显著减轻。（p<0.001）。与MTX联合治疗可显着减少MTX诱导的DNA损伤。
使用彗星方法研究RMLT对MTX引起的DNA损伤的可能保护作用。RMLT处理组的DNA损伤与MTX组相比显著减轻。（p<0.001）。与MTX联合治疗可显着减少MTX诱导的DNA损伤。
