PDF(Pubmed)
Abstract：
Oyster reefs are hotspots of denitrification mediated removal of dissolved nitrogen (N), however, information on their denitrifier microbiota is scarce. Furthermore, in oyster aquaculture, triploids are often preferred over diploids, yet again, microbiome differences between oyster ploidies are unknown. To address these knowledge gaps, farmed diploid and triploid oysters were collected over an annual growth cycle and analyzed using shotgun metagenomics and quantitative microbial elemental cycling (QMEC) techniques. Regardless of ploidy, Psychrobacter genus was abundant, with positive correlations found for genes of central metabolism, DNA metabolism, and carbohydrate metabolism. MAGs (metagenome-assembled genomes) yielded multiple Psychrobacter genomes harboring norB, narH, narI, and nirK denitrification genes, indicating their functional relevance within the eastern oysters. QMEC analysis indicated the predominance of carbon (C) and nitrogen (N) cycling genes, with no discernable patterns between ploidies. Among the N-cycling genes, the nosZII clade was overrepresented, suggesting its role in the eastern oyster\'s N removal processes.
摘要：
牡蛎礁是反硝化介导的溶解氮（N）去除的热点，然而,关于它们的反硝化微生物群的信息很少。此外，在牡蛎水产养殖中，三倍体往往优于二倍体，再一次,牡蛎倍性之间的微生物组差异未知。为了解决这些知识差距，在一个年度生长周期中收集养殖的二倍体和三倍体牡蛎，并使用shot弹枪宏基因组学和定量微生物元素循环（QMEC）技术进行分析。不管倍性如何，嗜冷杆菌属丰富，与中枢代谢基因的正相关，DNA代谢,和碳水化合物代谢。MAG（宏基因组组装的基因组）产生了多个携带norB的嗜冷杆菌基因组，narh,nari,和nirK反硝化基因，表明它们在东部牡蛎中的功能相关性。QMEC分析表明碳（C）和氮（N）循环基因占优势，倍性之间没有可辨别的模式。在N循环基因中，NosZII进化枝的比例过高,表明它在东部牡蛎氮去除过程中的作用。
