PDF(Pubmed)
Abstract：
Improved understanding of the complex interaction between plant metabolism, environmental conditions and the plant-associated microbiome requires an interdisciplinary approach: Our hypothesis in our multiomics study posited that several environmental and biotic factors have modulating effects on the microbiome and metabolome of the roots of wild Echium vulgare plants. Furthermore, we postulated reciprocal interactions between the root metabolome and microbiome. We investigated the metabolic content, the genetic variability, and the prokaryotic microbiome in the root systems of wild E. vulgare plants at rosette and flowering stages across six distinct locations. We incorporated the assessment of soil microbiomes and the measurement of selected soil chemical composition factors. Two distinct genetic clusters were determined based on microsatellite analysis without a consistent alignment with the geographical proximity between the locations. The microbial diversity of both the roots of E. vulgare and the surrounding bulk soil exhibited significant divergence across locations, varying soil pH characteristics, and within the identified plant genetic clusters. Notably, acidophilic bacteria were characteristic inhabitants of both soil and roots under acidic soil conditions, emphasizing the close interconnectedness between these compartments. The metabolome of E. vulgare significantly differed between root samples from different developmental stages, geographical locations, and soil pH levels. The developmental stage was the dominant driver of metabolome changes, with significantly higher concentrations of sugars, pyrrolizidine alkaloids, and some of their precursors in rosette stage plant roots. Our study featured the complex dynamics between soil pH, plant development, geographical locations, plant genetics, plant metabolome and microbiome, shedding light on existing knowledge gaps.
摘要：
提高了对植物代谢之间复杂相互作用的理解，环境条件和植物相关的微生物组需要跨学科的方法：我们在多组学研究中的假设认为，几种环境和生物因素对野生Echiumvulgare植物根部的微生物组和代谢组有调节作用。此外，我们假设根代谢组和微生物组之间的相互作用。我们调查了代谢含量，遗传变异，以及六个不同位置的莲座叶和开花期野生E.vulgare植物根系中的原核微生物组。我们纳入了土壤微生物组的评估和选定土壤化学成分因子的测量。基于微卫星分析确定了两个不同的遗传簇，而与位置之间的地理接近度没有一致的对齐。E.vulgare的根部和周围的块状土壤的微生物多样性在不同位置之间表现出明显的差异，不同的土壤pH特征，在确定的植物遗传集群内。值得注意的是,在酸性土壤条件下，嗜酸菌是土壤和根系的特征居民，强调这些隔室之间的紧密互联性。E.vulgare的代谢组在不同发育阶段的根样本之间显着不同，地理位置,和土壤pH值。发育阶段是代谢组变化的主要驱动因素，糖的浓度明显更高，吡咯啶生物碱，以及它们在玫瑰花期植物根中的一些前体。我们的研究以土壤pH值之间的复杂动力学为特征，植物发育,地理位置,植物遗传学,植物代谢组和微生物组，揭示现有的知识差距。
