PDF(Pubmed)
Abstract：
UNASSIGNED: Recent studies reported a link between high salt diet (HSD) and clinical exacerbation in mouse models of autoimmune diseases, mainly through the induction of pathogenic Th17 cells and/or HSD-induced dysbiosis. However, the topic remains controversial and not fully understood.
UNASSIGNED: In this study, we investigated the effects of HSD on the development of experimental autoimmune uveitis (EAU) in C57BL/6J mice.
UNASSIGNED: Unexpectedly, our data showed a significant attenuating effect of HSD on disease severity of native EAU, induced by direct immunization with IRBP peptide. That said, HSD had no effect on EAU disease severity induced by adoptive transfer of semi-purified auto-reactive IRBP-specific T lymphocytes. Accordingly, HSD did not affect IRBP-specific systemic afferent immune response as attested by no HSD-linked changes in T lymphocytes proliferation, cytokine production and Treg proportion. Gut microbiota analysis from cecal samples in naïve and EAU mice demonstrated that HSD affected differentially α-diversity between groups, whereas β-diversity was significantly modified in all groups. Unknown Tannerellaceae was the only taxon associated to HSD exposure in all treatment groups. Interestingly, a significantly higher abundance of unknown Gastranaerophilales, with potential anti-inflammatory properties, appeared in HSD-fed native EAU mice, only.
UNASSIGNED: In conclusion, our study suggests a possible impact of HSD on gut microbiota composition and consequently on development and clinical severity of EAU. Further studies are required to investigate the potential beneficial role of Gastranaerophilales in EAU.
UNASSIGNED: In this study, we investigated the effects of HSD on the development of experimental autoimmune uveitis (EAU) in C57BL/6J mice.
UNASSIGNED: Unexpectedly, our data showed a significant attenuating effect of HSD on disease severity of native EAU, induced by direct immunization with IRBP peptide. That said, HSD had no effect on EAU disease severity induced by adoptive transfer of semi-purified auto-reactive IRBP-specific T lymphocytes. Accordingly, HSD did not affect IRBP-specific systemic afferent immune response as attested by no HSD-linked changes in T lymphocytes proliferation, cytokine production and Treg proportion. Gut microbiota analysis from cecal samples in naïve and EAU mice demonstrated that HSD affected differentially α-diversity between groups, whereas β-diversity was significantly modified in all groups. Unknown Tannerellaceae was the only taxon associated to HSD exposure in all treatment groups. Interestingly, a significantly higher abundance of unknown Gastranaerophilales, with potential anti-inflammatory properties, appeared in HSD-fed native EAU mice, only.
UNASSIGNED: In conclusion, our study suggests a possible impact of HSD on gut microbiota composition and consequently on development and clinical severity of EAU. Further studies are required to investigate the potential beneficial role of Gastranaerophilales in EAU.
摘要：
■最近的研究报道了高盐饮食（HSD）与自身免疫性疾病小鼠模型的临床恶化之间的联系，主要通过致病性Th17细胞的诱导和/或HSD诱导的菌群失调。然而,这个话题仍然有争议，没有完全理解。
■在这项研究中，我们研究了HSD对C57BL/6J小鼠实验性自身免疫性葡萄膜炎（EAU）发展的影响。
■出乎意料的是，我们的数据显示HSD对天然EAU疾病严重程度的显著减弱作用，用IRBP肽直接免疫诱导。那就是说,HSD对过继转移半纯化的自身反应性IRBP特异性T淋巴细胞诱导的EAU疾病严重程度没有影响。因此，HSD不影响IRBP特异性全身传入免疫反应，如T淋巴细胞增殖无HSD相关变化所证明，细胞因子产生和Treg比例。来自未处理和EAU小鼠的盲肠样本的肠道微生物群分析表明，HSD影响组间差异α-多样性，而β-多样性在所有组中都显著改变。未知的制糖菌科是所有治疗组中唯一与HSD暴露相关的分类单元。有趣的是,大量的未知嗜血杆菌，具有潜在的抗炎特性，出现在HSD喂养的天然EAU小鼠中，only.
■总而言之，我们的研究提示HSD可能对肠道菌群组成产生影响,进而对EAU的发展和临床严重程度产生影响.需要进一步的研究来研究嗜血杆菌在EAU中的潜在有益作用。
■在这项研究中，我们研究了HSD对C57BL/6J小鼠实验性自身免疫性葡萄膜炎（EAU）发展的影响。
■出乎意料的是，我们的数据显示HSD对天然EAU疾病严重程度的显著减弱作用，用IRBP肽直接免疫诱导。那就是说,HSD对过继转移半纯化的自身反应性IRBP特异性T淋巴细胞诱导的EAU疾病严重程度没有影响。因此，HSD不影响IRBP特异性全身传入免疫反应，如T淋巴细胞增殖无HSD相关变化所证明，细胞因子产生和Treg比例。来自未处理和EAU小鼠的盲肠样本的肠道微生物群分析表明，HSD影响组间差异α-多样性，而β-多样性在所有组中都显著改变。未知的制糖菌科是所有治疗组中唯一与HSD暴露相关的分类单元。有趣的是,大量的未知嗜血杆菌，具有潜在的抗炎特性，出现在HSD喂养的天然EAU小鼠中，only.
■总而言之，我们的研究提示HSD可能对肠道菌群组成产生影响,进而对EAU的发展和临床严重程度产生影响.需要进一步的研究来研究嗜血杆菌在EAU中的潜在有益作用。
