PDF(Pubmed)
Abstract：
UNASSIGNED: Acute myeloid leukaemia (AML) is a disease of the haematopoietic stem cells(HSCs) that is characterised by the uncontrolled proliferation and impaired differentiation of normal haematopoietic stem/progenitor cells. Several pathways that control the proliferation and differentiation of HSCs are impaired in AML. Activation of the Wnt/beta-catenin signalling pathway has been shown in AML and beta-catenin, which is thought to be the key element of this pathway, has been frequently highlighted. The present study was designed to determine beta-catenin expression levels and beta-catenin-related genes in AML.
UNASSIGNED: In this study, beta-catenin gene expression levels were determined in 19 AML patients and 3 controls by qRT-PCR. Transcriptome analysis was performed on AML grouped according to beta-catenin expression levels. Differentially expressed genes(DEGs) were investigated in detail using the Database for Annotation Visualisation and Integrated Discovery(DAVID), Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes(KEGG), STRING online tools.
UNASSIGNED: The transcriptome profiles of our AML samples showed different molecular signature profiles according to their beta-catenin levels(high-low). A total of 20 genes have been identified as hub genes. Among these, TTK, HJURP, KIF14, BTF3, RPL17 and RSL1D1 were found to be associated with beta-catenin and poor survival in AML. Furthermore, for the first time in our study, the ELOV6 gene, which is the most highly up-regulated gene in human AML samples, was correlated with a poor prognosis via high beta-catenin levels.
UNASSIGNED: It is suggested that the identification of beta-catenin-related gene profiles in AML may help to select new therapeutic targets for the treatment of AML.
UNASSIGNED: In this study, beta-catenin gene expression levels were determined in 19 AML patients and 3 controls by qRT-PCR. Transcriptome analysis was performed on AML grouped according to beta-catenin expression levels. Differentially expressed genes(DEGs) were investigated in detail using the Database for Annotation Visualisation and Integrated Discovery(DAVID), Gene Ontology(GO), Kyoto Encyclopedia of Genes and Genomes(KEGG), STRING online tools.
UNASSIGNED: The transcriptome profiles of our AML samples showed different molecular signature profiles according to their beta-catenin levels(high-low). A total of 20 genes have been identified as hub genes. Among these, TTK, HJURP, KIF14, BTF3, RPL17 and RSL1D1 were found to be associated with beta-catenin and poor survival in AML. Furthermore, for the first time in our study, the ELOV6 gene, which is the most highly up-regulated gene in human AML samples, was correlated with a poor prognosis via high beta-catenin levels.
UNASSIGNED: It is suggested that the identification of beta-catenin-related gene profiles in AML may help to select new therapeutic targets for the treatment of AML.
摘要：
急性骨髓性白血病(AML)是造血干细胞(HSC)的疾病,其特征在于正常造血干/祖细胞的不受控制的增殖和受损的分化。在AML中，控制HSC增殖和分化的几种途径受损。Wnt/β-catenin信号通路的激活已在AML和β-catenin中显示，这被认为是这一途径的关键要素，经常被强调。本研究旨在确定AML中β-连环蛋白的表达水平和β-连环蛋白相关基因。
■在这项研究中，通过qRT-PCR测定19例AML患者和3例对照中的β-连环蛋白基因表达水平。对根据β-连环蛋白表达水平分组的AML进行转录组分析。差异表达基因（DEGs）使用注释可视化和集成发现数据库（DAVID）进行了详细研究，基因本体论（GO），京都基因和基因组百科全书（KEGG），STRING在线工具。
■我们的AML样品的转录组图谱根据其β-连环蛋白水平（高-低）显示出不同的分子特征谱。总共有20个基因被鉴定为hub基因。其中,TTK,HJURP,发现KIF14、BTF3、RPL17和RSL1D1与β-连环蛋白和AML的低生存率相关。此外，在我们的研究中第一次，ELOV6基因，这是人类AML样本中最高度上调的基因，通过高β-连环蛋白水平与不良预后相关。
■提示AML中β-连环蛋白相关基因谱的鉴定可能有助于选择治疗AML的新治疗靶标。
■在这项研究中，通过qRT-PCR测定19例AML患者和3例对照中的β-连环蛋白基因表达水平。对根据β-连环蛋白表达水平分组的AML进行转录组分析。差异表达基因（DEGs）使用注释可视化和集成发现数据库（DAVID）进行了详细研究，基因本体论（GO），京都基因和基因组百科全书（KEGG），STRING在线工具。
■我们的AML样品的转录组图谱根据其β-连环蛋白水平（高-低）显示出不同的分子特征谱。总共有20个基因被鉴定为hub基因。其中,TTK,HJURP,发现KIF14、BTF3、RPL17和RSL1D1与β-连环蛋白和AML的低生存率相关。此外，在我们的研究中第一次，ELOV6基因，这是人类AML样本中最高度上调的基因，通过高β-连环蛋白水平与不良预后相关。
■提示AML中β-连环蛋白相关基因谱的鉴定可能有助于选择治疗AML的新治疗靶标。
