PDF(Pubmed)
Abstract：
Senecavirus A (SVA) is an emerging virus that causes the vesicular disease in pigs, clinically indistinguishable from other high consequence vesicular diseases. This virus belongs to the genus Senecavirus in the family Picornaviridae. Its genome is a positive-sense, single-stranded RNA, approximately 7,300 nt in length, with a 3\' poly(A) tail but without 5\'-end capped structure. SVA can efficiently propagate in different cells, including some non-pig-derived cell lines. A wild-type SVA was previously rescued from its cDNA clone using reverse genetics in our laboratory. In the present study, the BSR-T7/5 cell line was inoculated with the passage-5 SVA. At 12 h post-inoculation, SVA-infected and non-infected cells were independently collected for the analysis on comparative transcriptomics. The results totally showed 628 differentially expressed genes, including 565 upregulated and 63 downregulated ones, suggesting that SVA infection significantly stimulated the transcription initiation in cells. GO and KEGG enrichment analyses demonstrated that SVA exerted multiple effects on immunity-related pathways in cells. Furthermore, the RNA sequencing data were subjected to other in-depth analyses, such as the single-nucleotide polymorphism, transcription factors, and protein-protein interactions. The present study, along with our previous proteomics and metabolomics researches, provides a multi-omics insight into the interaction between SVA and its hosts.
摘要：
SenecavirusA（SVA）是一种引起猪水泡病的新兴病毒，临床上与其他高后果的水疱性疾病难以区分。该病毒属于小牛科Senecavirus属。它的基因组是一个积极意义，单链RNA，长度约为7,300nt，具有3\'聚(A)尾,但没有5\'端封端结构。SVA可以在不同的细胞中有效繁殖，包括一些非猪来源的细胞系。先前在我们的实验室中使用反向遗传学从其cDNA克隆中拯救了野生型SVA。在本研究中,用传代-5SVA接种BSR-T7/5细胞系。接种后12小时，独立收集SVA感染和未感染的细胞用于比较转录组学分析。结果显示共628个差异表达基因,包括565个上调和63个下调，表明SVA感染显著刺激细胞中的转录起始。GO和KEGG富集分析表明SVA对细胞中免疫相关途径发挥多种作用。此外，RNA测序数据进行了其他深入分析，比如单核苷酸多态性，转录因子，和蛋白质-蛋白质相互作用。本研究,随着我们以前的蛋白质组学和代谢组学研究，提供了对SVA与其宿主之间相互作用的多组学洞察。
