PDF(Pubmed)
Abstract：
UNASSIGNED: Idiopathic epilepsy (IE) and meningoencephalomyelitis of unknown origin (MUO) are common causes of brain diseases leading to seizures in dogs. In this study, the concentrations of 196 lipid metabolites and nitrogen oxide (NO) production in the cerebrospinal fluid (CSF) and plasma of dogs with MUO or IE were measured using a LC-MS/MS and a NOx analyzer, respectively.
UNASSIGNED: Nine clinically healthy dogs and 11 and 12 dogs with IE and MUO, respectively, were included in the study.
UNASSIGNED: Lipid analysis revealed variations in the levels of four and six lipid metabolites in CSF and plasma, respectively, between the groups. The levels of 6-keto-prostaglandin (PG) F1α (PGF1α), 20-carboxy arachidonic acid (20-carboxy-AA), 9-hydroxyoctadecadienoic acid, and lyso-platelet-activating factor were high in the CSF of dogs with MUO. In addition, the plasma levels of 11,12-dihydroxyeicosatrienoic acid, 20-carboxy-AA, and oleoylethanolamide were high in dogs with IE, and those of PGF1α were high in dogs with MUO. NO production levels were high in CSF but not in plasma in dogs with MUO or IE.
UNASSIGNED: It remains unknown whether these changes represent the cause or effect of diseases of the central nervous system; however, lipid metabolites and NO production in CSF and plasma may be used as diagnostic biomarkers and could be exploited for treating idiopathic or inflammatory epilepsy in dogs.
UNASSIGNED: Nine clinically healthy dogs and 11 and 12 dogs with IE and MUO, respectively, were included in the study.
UNASSIGNED: Lipid analysis revealed variations in the levels of four and six lipid metabolites in CSF and plasma, respectively, between the groups. The levels of 6-keto-prostaglandin (PG) F1α (PGF1α), 20-carboxy arachidonic acid (20-carboxy-AA), 9-hydroxyoctadecadienoic acid, and lyso-platelet-activating factor were high in the CSF of dogs with MUO. In addition, the plasma levels of 11,12-dihydroxyeicosatrienoic acid, 20-carboxy-AA, and oleoylethanolamide were high in dogs with IE, and those of PGF1α were high in dogs with MUO. NO production levels were high in CSF but not in plasma in dogs with MUO or IE.
UNASSIGNED: It remains unknown whether these changes represent the cause or effect of diseases of the central nervous system; however, lipid metabolites and NO production in CSF and plasma may be used as diagnostic biomarkers and could be exploited for treating idiopathic or inflammatory epilepsy in dogs.
摘要：
■特发性癫痫（IE）和不明原因的脑膜脑脊髓炎（MUO）是导致狗癫痫发作的脑部疾病的常见原因。在这项研究中,使用LC-MS/MS和NOx分析仪测量MUO或IE犬的脑脊液（CSF）和血浆中196种脂质代谢物和氮氧化物（NO）的浓度，分别。
■九只临床健康的狗以及11和12只IE和MUO的狗，分别,包括在研究中。
■脂质分析显示CSF和血浆中四种和六种脂质代谢产物的水平变化，分别,群体之间。6-酮-前列腺素(PG)F1α(PGF1α)的水平,20-羧基花生四烯酸（20-羧基-AA），9-羟基十八碳二烯酸，MUO犬的脑脊液中溶血血小板活化因子高。此外,11,12-二羟基二十碳三烯酸的血浆水平，20-羧基-AA,油酰乙醇胺在IE犬中含量高，MUO犬的PGF1α含量较高。在患有MUO或IE的狗中，CSF中NO的产生水平很高，但血浆中NO的产生水平却不高。
■这些变化是否代表中枢神经系统疾病的原因或影响尚不清楚；但是，CSF和血浆中的脂质代谢产物和NO的产生可用作诊断生物标志物，可用于治疗犬的特发性或炎症性癫痫。
■九只临床健康的狗以及11和12只IE和MUO的狗，分别,包括在研究中。
■脂质分析显示CSF和血浆中四种和六种脂质代谢产物的水平变化，分别,群体之间。6-酮-前列腺素(PG)F1α(PGF1α)的水平,20-羧基花生四烯酸（20-羧基-AA），9-羟基十八碳二烯酸，MUO犬的脑脊液中溶血血小板活化因子高。此外,11,12-二羟基二十碳三烯酸的血浆水平，20-羧基-AA,油酰乙醇胺在IE犬中含量高，MUO犬的PGF1α含量较高。在患有MUO或IE的狗中，CSF中NO的产生水平很高，但血浆中NO的产生水平却不高。
■这些变化是否代表中枢神经系统疾病的原因或影响尚不清楚；但是，CSF和血浆中的脂质代谢产物和NO的产生可用作诊断生物标志物，可用于治疗犬的特发性或炎症性癫痫。
