PDF(Pubmed)
Abstract：
Differential expression of genes is key to mediating developmental and stress-related plant responses. Here, we addressed the regulation of plant metabolic responses to biotic stress and the developmental variation of defense-related genes in four species of the genus Datura with variable patterns of metabolite accumulation and development. We combine transcriptome profiling with phylogenomic techniques to analyze gene expression and coexpression in plants subjected to damage by a specialist folivore insect. We found (1) common overall gene expression in species of similar chemical profiles, (2) species-specific responses of proteins involved in specialized metabolism, characterized by constant levels of gene expression coupled with transcriptional rearrangement, and (3) induction of transcriptional rearrangement of major terpene and tropane alkaloid genes upon herbivory. Our results indicate differential modulation of terpene and tropane metabolism linked to jasmonate signaling and specific transcription factors to regulate developmental variation and stress programs, and suggest plastic adaptive responses to cope with herbivory. The transcriptional profiles of specialized metabolism shown here reveal complex genetic control of plant metabolism and contribute to understanding the molecular basis of adaptations and the physiological variation of significant ecological traits.
摘要：
基因的差异表达是介导发育和胁迫相关植物反应的关键。这里,我们研究了曼陀罗属四个物种中植物对生物胁迫的代谢反应的调节以及防御相关基因的发育变异，这些物种具有不同的代谢物积累和发育模式。我们将转录组分析与系统基因组技术相结合，以分析遭受特殊folivore昆虫破坏的植物中的基因表达和共表达。我们发现（1）在相似化学谱的物种中共同的整体基因表达，（2）参与特殊代谢的蛋白质的物种特异性反应，以恒定水平的基因表达和转录重排为特征，（3）草食动物诱导主要萜烯和托烷生物碱基因的转录重排。我们的结果表明，与茉莉酸信号和特定转录因子相关的萜烯和托烷代谢的差异调节，以调节发育变异和应激程序，并建议塑料适应性反应来应对食草动物。此处显示的专门代谢的转录谱揭示了植物代谢的复杂遗传控制，并有助于理解适应的分子基础和重要生态性状的生理变异。
