PDF(Pubmed)
Abstract：
UNASSIGNED: Coronary artery disease (CAD) is a debilitating condition that can lead to myocardial infarction (MI). Exosomal miRNAs (exo-miRNA) can be diagnostic biomarkers for detecting MI. Here, we conduct a study to evaluate the efficacy of exo-miRNA-21-5p/3p for early detection of MI.
UNASSIGNED: A total of 135 CAD patients and 150 healthy subjects participated in this study. Additionally, we randomly divided 26 male Wistar rats (12 weeks old) into two groups: control and induced MI. Angiographic images were used to identify patients and healthy individuals of all genders. In the following, serum exosomes were obtained, and exo-miRNA-21-5p/3p was measured by reverse-transcriptase polymerase chain reaction.
UNASSIGNED: We observed an upregulation of exo-miRNA-21-5p/3p in CAD patient and MI-induced animal groups compared to controls. Analysis of the ROC curves defined 82% and 88% of the participants\' exo-miRNA-21-5p and exo-miRNA-21-3p diagnostic power, respectively, which in the animal model was 92 and 82.
UNASSIGNED: This study revealed that the mean expression levels of exo-miRNA-21-5p/3p were significantly increased in CAD patients and animal models of induced MI. Also, these results are associated with the atherogenic lipid profile of CAD patients, which may play an important role in the progression of the disease. Therefore, they can be considered as novel biomarkers.
UNASSIGNED: A total of 135 CAD patients and 150 healthy subjects participated in this study. Additionally, we randomly divided 26 male Wistar rats (12 weeks old) into two groups: control and induced MI. Angiographic images were used to identify patients and healthy individuals of all genders. In the following, serum exosomes were obtained, and exo-miRNA-21-5p/3p was measured by reverse-transcriptase polymerase chain reaction.
UNASSIGNED: We observed an upregulation of exo-miRNA-21-5p/3p in CAD patient and MI-induced animal groups compared to controls. Analysis of the ROC curves defined 82% and 88% of the participants\' exo-miRNA-21-5p and exo-miRNA-21-3p diagnostic power, respectively, which in the animal model was 92 and 82.
UNASSIGNED: This study revealed that the mean expression levels of exo-miRNA-21-5p/3p were significantly increased in CAD patients and animal models of induced MI. Also, these results are associated with the atherogenic lipid profile of CAD patients, which may play an important role in the progression of the disease. Therefore, they can be considered as novel biomarkers.
摘要：
■冠状动脉疾病（CAD）是一种使人衰弱的疾病，可导致心肌梗塞（MI）。外来体miRNA(exo-miRNA)可以是用于检测MI的诊断生物标志物。这里,我们进行了一项研究,以评估exo-miRNA-21-5p/3p早期检测MI的功效.
■共有135名CAD患者和150名健康受试者参与了这项研究。此外,我们将26只雄性Wistar大鼠（12周龄）随机分为两组：对照组和诱发MI。使用血管造影图像来识别所有性别的患者和健康个体。在下文中，获得血清外泌体，和exo-miRNA-21-5p/3p通过逆转录酶聚合酶链反应测量。
我们观察到与对照相比,在CAD患者和MI诱导的动物组中exo-miRNA-21-5p/3p的上调。ROC曲线分析定义了82%和88%的参与者exo-miRNA-21-5p和exo-miRNA-21-3p诊断能力，分别,在动物模型中分别为92和82。
■该研究揭示,在CAD患者和诱发MI的动物模型中,exo-miRNA-21-5p/3p的平均表达水平显著增加。此外，这些结果与CAD患者的动脉粥样硬化脂质分布有关，这可能在疾病的进展中起重要作用。因此,它们可以被认为是新的生物标志物。
■共有135名CAD患者和150名健康受试者参与了这项研究。此外,我们将26只雄性Wistar大鼠（12周龄）随机分为两组：对照组和诱发MI。使用血管造影图像来识别所有性别的患者和健康个体。在下文中，获得血清外泌体，和exo-miRNA-21-5p/3p通过逆转录酶聚合酶链反应测量。
我们观察到与对照相比,在CAD患者和MI诱导的动物组中exo-miRNA-21-5p/3p的上调。ROC曲线分析定义了82%和88%的参与者exo-miRNA-21-5p和exo-miRNA-21-3p诊断能力，分别,在动物模型中分别为92和82。
■该研究揭示,在CAD患者和诱发MI的动物模型中,exo-miRNA-21-5p/3p的平均表达水平显著增加。此外，这些结果与CAD患者的动脉粥样硬化脂质分布有关，这可能在疾病的进展中起重要作用。因此,它们可以被认为是新的生物标志物。
