PDF(Pubmed)
Abstract：
Dental caries is a common human oral disease worldwide, caused by an acid-producing bacteria Streptococcus mutans. The use of synthetic drugs and antibiotics to prevent dental caries has been increasing, but this can lead to severe side effects. To solve this issue, developing and developed countries have resorted to herbal medicines as an alternative to synthetic drugs for the treatment and prevention of dental caries. Therefore, there is an urgent need for plant-derived products to treat such diseases. Bacopa monnieri, a well-documented medicinal plant, contains 52 phytocompounds, including the pentacyclic triterpenoid metabolite known as asiatic acid (ASTA). Hence, this study aimed to demonstrate, for the first time, the antibacterial activity of phytocompound ASTA against S. mutans. The findings revealed that ASTA significantly inhibited the growth of S. mutans and the production of virulence factors such as acidurity, acidogenicity, and eDNA synthesis. Molecular docking analysis evaluated the potential activity of ASTA against S. mutans virulence genes, including VicR and GtfC. Furthermore, toxicity assessment of ASTA in human buccal epithelial cells was performed, and no morphological changes were observed. An in vivo analysis using Danio rerio (zebrafish) confirmed that the ASTA treatment significantly increased the survival rates of infected fish by hindering the intestinal colonization of S. mutans. Furthermore, the disease protection potential of ASTA against the pathognomonic symptom of S. mutans infection was proven by the histopathological examination of the gills, gut, and kidney. Overall, these findings suggest that ASTA may be a promising therapeutic and alternative drug for the treatment and prevention of oral infection imposed by S. mutans.
摘要：
龋齿是世界范围内人类常见的口腔疾病,由产酸细菌变形链球菌引起的。预防龋齿的合成药物和抗生素的使用一直在增加,但这会导致严重的副作用.为了解决这个问题，发展中国家和发达国家已将草药作为合成药物的替代品，用于治疗和预防龋齿。因此,迫切需要植物衍生产品来治疗这些疾病。BacopaMonnieri,一种有据可查的药用植物，含有52种植物化合物，包括称为积雪草酸(ASTA)的五环三萜类代谢物。因此，这项研究旨在证明，第一次,植物化合物ASTA对变形链球菌的抗菌活性。研究结果表明，ASTA显著抑制变异链球菌的生长和酸性等毒力因子的产生，酸化，和eDNA合成。分子对接分析评估了ASTA对变异链球菌毒力基因的潜在活性，包括VicR和GtfC.此外，对人口腔上皮细胞进行ASTA的毒性评估，未观察到形态学变化。使用Daniorerio（斑马鱼）的体内分析证实，ASTA处理通过阻碍变形链球菌的肠道定植，显着提高了感染鱼的存活率。此外，ASTA对变异链球菌感染的病理症状的疾病保护潜力已通过g的组织病理学检查得到证实，gut,gut还有肾.总的来说,这些研究结果表明,ASTA可能是治疗和预防变形链球菌口腔感染的一种有前景的治疗和替代药物.
