PDF(Pubmed)
Abstract：
UNASSIGNED: The mainstay of treatment for COVID-19-associated mucormycosis was liposomal Amphotericin B. Other antifungal agents, such as posaconazole and isavuconazole, were used as well. The Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antimicrobial Susceptibility Testing recommend broth microdilution methods for antifungal susceptibility testing. In this regard, the present study aimed to see what potency and zone diameters correlate with the gold standard broth microdilution method.
UNASSIGNED: All the isolates were identified by matrix-assisted laser desorption ionization-time-of-flight. In total, 127 isolates of 83 Rhizopus oryzae complex and 44 isolates of Rhizopus microsporus complex were selected. Anti-fungal susceptibility testing by disc diffusion and E-test was performed on Mueller Hinton Agar and compared with the CLSI broth microdilution method of Anti-fungal susceptibility testing.
UNASSIGNED: Percentage agreement was found to be more in the case of the E test than the disc diffusion method. In the case of R. oryzae, posaconazole had 98.79% agreement with broth microdilution followed by Isavuconazole (97.59%), Itraconazole (96.38%), and Amphotericin B (91.56%).
UNASSIGNED: Disc diffusion correlates well with broth microdilution, although its correlation is weaker when compared to the E test. Effective concentration of Amphotericin B discs for antifungal susceptibility testing depends on the specific Rhizopus species.
UNASSIGNED: All the isolates were identified by matrix-assisted laser desorption ionization-time-of-flight. In total, 127 isolates of 83 Rhizopus oryzae complex and 44 isolates of Rhizopus microsporus complex were selected. Anti-fungal susceptibility testing by disc diffusion and E-test was performed on Mueller Hinton Agar and compared with the CLSI broth microdilution method of Anti-fungal susceptibility testing.
UNASSIGNED: Percentage agreement was found to be more in the case of the E test than the disc diffusion method. In the case of R. oryzae, posaconazole had 98.79% agreement with broth microdilution followed by Isavuconazole (97.59%), Itraconazole (96.38%), and Amphotericin B (91.56%).
UNASSIGNED: Disc diffusion correlates well with broth microdilution, although its correlation is weaker when compared to the E test. Effective concentration of Amphotericin B discs for antifungal susceptibility testing depends on the specific Rhizopus species.
摘要：
■COVID-19相关性毛霉菌病的主要治疗方法是脂质体两性霉素B。其他抗真菌药物，如泊沙康唑和伊沙康康唑，也使用过。临床和实验室标准研究所（CLSI）和欧洲抗菌药物敏感性测试委员会推荐了用于抗真菌药物敏感性测试的微量肉汤稀释方法。在这方面,本研究旨在了解金标准肉汤微量稀释法的效力和区域直径。
■通过基质辅助激光解吸电离-飞行时间鉴定所有分离物。总的来说,选择了127个分离株的83个米根霉复合物和44个分离株的小孢子根霉复合物。通过圆盘扩散和E测试对MuellerHinton琼脂进行抗真菌药敏试验，并与抗真菌药敏试验的CLSI肉汤微量稀释方法进行比较。
■在E测试的情况下，发现百分比一致性比圆盘扩散方法更多。在米曲霉的情况下，泊沙康唑与肉汤微量稀释有98.79%的一致性，其次是伊沙康康唑(97.59%)，伊曲康唑（96.38%），两性霉素B(91.56%)。
■圆盘扩散与肉汤微量稀释度密切相关，尽管与E检验相比，其相关性较弱。用于抗真菌药敏试验的两性霉素B圆盘的有效浓度取决于具体的根霉种类。
■通过基质辅助激光解吸电离-飞行时间鉴定所有分离物。总的来说,选择了127个分离株的83个米根霉复合物和44个分离株的小孢子根霉复合物。通过圆盘扩散和E测试对MuellerHinton琼脂进行抗真菌药敏试验，并与抗真菌药敏试验的CLSI肉汤微量稀释方法进行比较。
■在E测试的情况下，发现百分比一致性比圆盘扩散方法更多。在米曲霉的情况下，泊沙康唑与肉汤微量稀释有98.79%的一致性，其次是伊沙康康唑(97.59%)，伊曲康唑（96.38%），两性霉素B(91.56%)。
■圆盘扩散与肉汤微量稀释度密切相关，尽管与E检验相比，其相关性较弱。用于抗真菌药敏试验的两性霉素B圆盘的有效浓度取决于具体的根霉种类。
