UNASSIGNED: All the isolates were identified by matrix-assisted laser desorption ionization-time-of-flight. In total, 127 isolates of 83 Rhizopus oryzae complex and 44 isolates of Rhizopus microsporus complex were selected. Anti-fungal susceptibility testing by disc diffusion and E-test was performed on Mueller Hinton Agar and compared with the CLSI broth microdilution method of Anti-fungal susceptibility testing.
UNASSIGNED: Percentage agreement was found to be more in the case of the E test than the disc diffusion method. In the case of R. oryzae, posaconazole had 98.79% agreement with broth microdilution followed by Isavuconazole (97.59%), Itraconazole (96.38%), and Amphotericin B (91.56%).
UNASSIGNED: Disc diffusion correlates well with broth microdilution, although its correlation is weaker when compared to the E test. Effective concentration of Amphotericin B discs for antifungal susceptibility testing depends on the specific Rhizopus species.
■通过基质辅助激光解吸电离-飞行时间鉴定所有分离物。总的来说,选择了127个分离株的83个米根霉复合物和44个分离株的小孢子根霉复合物。通过圆盘扩散和E测试对MuellerHinton琼脂进行抗真菌药敏试验,并与抗真菌药敏试验的CLSI肉汤微量稀释方法进行比较。
■在E测试的情况下,发现百分比一致性比圆盘扩散方法更多。在米曲霉的情况下,泊沙康唑与肉汤微量稀释有98.79%的一致性,其次是伊沙康康唑(97.59%),伊曲康唑(96.38%),两性霉素B(91.56%)。
■圆盘扩散与肉汤微量稀释度密切相关,尽管与E检验相比,其相关性较弱。用于抗真菌药敏试验的两性霉素B圆盘的有效浓度取决于具体的根霉种类。