PDF(Pubmed)
Abstract：
UNASSIGNED: To investigate the role of the chemokines CXCL13, CXCL10 and CXCL8 in the diagnosis of ocular- and neurosyphilis by examining the serum, aqueous humour (AH) and cerebrospinal fluid (CSF) of patients with ocular syphilis.
UNASSIGNED: An observational descriptive study was performed prospectively at Tygerberg Academic Hospital in Cape Town, South Africa from 1 February 2018 till 31 January 2021 which enrolled 23 participants. 14 Patients were male and 9 female, 15 patients were HIV positive, and all patients were newly diagnosed with ocular syphilis. Upon diagnosis of ocular syphilis, the HIV status of each patient was determined, and 3 samples (AH, serum and CSF) were collected to measure the levels of CXCL13, CXCL10 and CXCL8 in each. All patients were treated with 14 days of intravenous Penicillin G and topical corticosteroid drops for uveitis.
UNASSIGNED: The mean concentrations of all 3 biomarkers were higher in the AH and CSF than in the serum. The mean concentrations of the 3 measured biomarkers were markedly different when comparing both AH and CSF levels to serum levels. The level of CXCL13 measured in the AH correlated well with the concentrations found in the CSF of patients with neurosyphilis. In patients with neurosyphilis, mean AH levels of CXCL13 and CXCL10 were markedly higher than in serum while mean CSF levels of CXCL10 were also markedly higher than in serum. Also, the AH/serum ratio of CXCL13 and CXCL10, as well as the CSF/serum ratio of CXCL10, was much higher in patients with neurosyphilis than without. In patients with HIV infection, mean AH CXCL13 levels were much higher than in patients without HIV infection.
UNASSIGNED: The levels of CXCL13, CXCL10 and CXCL8 in the AH of patients with neurosyphilis are similar to previously reported levels in the CSF of patients with neurosyphilis and can potentially be an adjunct in the diagnosis of ocular syphilis. Patients with ocular syphilis who tested negative for neurosyphilis with conventional CSF testing showed features of neurosyphilis when analysing the CSF chemokines.
UNASSIGNED: An observational descriptive study was performed prospectively at Tygerberg Academic Hospital in Cape Town, South Africa from 1 February 2018 till 31 January 2021 which enrolled 23 participants. 14 Patients were male and 9 female, 15 patients were HIV positive, and all patients were newly diagnosed with ocular syphilis. Upon diagnosis of ocular syphilis, the HIV status of each patient was determined, and 3 samples (AH, serum and CSF) were collected to measure the levels of CXCL13, CXCL10 and CXCL8 in each. All patients were treated with 14 days of intravenous Penicillin G and topical corticosteroid drops for uveitis.
UNASSIGNED: The mean concentrations of all 3 biomarkers were higher in the AH and CSF than in the serum. The mean concentrations of the 3 measured biomarkers were markedly different when comparing both AH and CSF levels to serum levels. The level of CXCL13 measured in the AH correlated well with the concentrations found in the CSF of patients with neurosyphilis. In patients with neurosyphilis, mean AH levels of CXCL13 and CXCL10 were markedly higher than in serum while mean CSF levels of CXCL10 were also markedly higher than in serum. Also, the AH/serum ratio of CXCL13 and CXCL10, as well as the CSF/serum ratio of CXCL10, was much higher in patients with neurosyphilis than without. In patients with HIV infection, mean AH CXCL13 levels were much higher than in patients without HIV infection.
UNASSIGNED: The levels of CXCL13, CXCL10 and CXCL8 in the AH of patients with neurosyphilis are similar to previously reported levels in the CSF of patients with neurosyphilis and can potentially be an adjunct in the diagnosis of ocular syphilis. Patients with ocular syphilis who tested negative for neurosyphilis with conventional CSF testing showed features of neurosyphilis when analysing the CSF chemokines.
摘要：
■通过检测血清来研究趋化因子CXCL13、CXCL10和CXCL8在诊断眼部和神经梅毒中的作用，眼梅毒患者的房水（AH）和脑脊液（CSF）。
■一项观察性描述性研究是在开普敦的Tygerberg学术医院进行的，南非从2018年2月1日至2021年1月31日，注册了23名参与者。男14例,女9例,15名患者为HIV阳性，所有患者均新诊断为眼部梅毒。在诊断为眼部梅毒时，确定了每位患者的艾滋病毒状况，和3个样本(AH，收集血清和CSF）以测量各自中CXCL13，CXCL10和CXCL8的水平。所有患者均接受14天静脉注射青霉素G和局部皮质类固醇滴剂治疗葡萄膜炎。
■AH和CSF中所有3种生物标志物的平均浓度高于血清。当将AH和CSF水平与血清水平进行比较时,3种测量的生物标志物的平均浓度显著不同。在AH中测得的CXCL13水平与神经梅毒患者CSF中的浓度密切相关。在神经梅毒患者中，CXCL13和CXCL10的平均AH水平明显高于血清,而CXCL10的平均CSF水平也明显高于血清.此外，神经梅毒患者的CXCL13和CXCL10的AH/血清比率以及CXCL10的CSF/血清比率远高于无神经梅毒患者。在HIV感染患者中，平均AHCXCL13水平远高于无HIV感染患者.
■神经梅毒患者AH中CXCL13，CXCL10和CXCL8的水平与先前报道的神经梅毒患者CSF中的水平相似，可能是诊断的辅助手段眼梅毒。在分析CSF趋化因子时，经常规CSF测试对神经梅毒呈阴性的眼部梅毒患者显示出神经梅毒的特征。
■一项观察性描述性研究是在开普敦的Tygerberg学术医院进行的，南非从2018年2月1日至2021年1月31日，注册了23名参与者。男14例,女9例,15名患者为HIV阳性，所有患者均新诊断为眼部梅毒。在诊断为眼部梅毒时，确定了每位患者的艾滋病毒状况，和3个样本(AH，收集血清和CSF）以测量各自中CXCL13，CXCL10和CXCL8的水平。所有患者均接受14天静脉注射青霉素G和局部皮质类固醇滴剂治疗葡萄膜炎。
■AH和CSF中所有3种生物标志物的平均浓度高于血清。当将AH和CSF水平与血清水平进行比较时,3种测量的生物标志物的平均浓度显著不同。在AH中测得的CXCL13水平与神经梅毒患者CSF中的浓度密切相关。在神经梅毒患者中，CXCL13和CXCL10的平均AH水平明显高于血清,而CXCL10的平均CSF水平也明显高于血清.此外，神经梅毒患者的CXCL13和CXCL10的AH/血清比率以及CXCL10的CSF/血清比率远高于无神经梅毒患者。在HIV感染患者中，平均AHCXCL13水平远高于无HIV感染患者.
■神经梅毒患者AH中CXCL13，CXCL10和CXCL8的水平与先前报道的神经梅毒患者CSF中的水平相似，可能是诊断的辅助手段眼梅毒。在分析CSF趋化因子时，经常规CSF测试对神经梅毒呈阴性的眼部梅毒患者显示出神经梅毒的特征。
