PDF(Pubmed)
Abstract：
UNASSIGNED: Pulmonary tuberculosis (PTB) remains a significant health concern, particularly in individuals infected with human immunodeficiency virus (HIV) who are more susceptible to developing active TB disease. Early and accurate diagnosis of TB is crucial for effective treatment and prevention of transmission. This study aims to evaluate the potential of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of bronchoalveolar lavage fluid (BALF) for diagnosis of suspected PTB in HIV-infected patients.
UNASSIGNED: This retrospective study recruited 60 HIV-infected patients with suspected PTB presenting with respiratory symptoms and abnormal chest radiographs between January 2022 and June 2023. BALF samples were collected and subjected to analysis using MALDI-TOF MS, GeneXpert, acid-fast bacilli (AFB) smear and culture. And their diagnostic performance was compared.
UNASSIGNED: The sensitivity of MALDI⁃TOFMS for diagnosing PTB was 83.3 %, which was better than that of smear 11.9 %, culture 40.5 % or Xpert38.1 % (all p < 0.01). The area under the curve (AUC) value of MALDI⁃TOFMS was 0.889, which was better than that of smear 0.532, culture 0.675 or Xpert 0.690 (all p < 0.01). The katG315 and rpoB-RRDR 511 mutations were detected by the MALDI⁃TOFMS in two patients.
UNASSIGNED: Nucleotide MALDI-TOFMS has a good clinical performance for rapid diagnosis of PTB from BALF samples in HIV infected patients, and detects mutations of TB simultaneously.
UNASSIGNED: This retrospective study recruited 60 HIV-infected patients with suspected PTB presenting with respiratory symptoms and abnormal chest radiographs between January 2022 and June 2023. BALF samples were collected and subjected to analysis using MALDI-TOF MS, GeneXpert, acid-fast bacilli (AFB) smear and culture. And their diagnostic performance was compared.
UNASSIGNED: The sensitivity of MALDI⁃TOFMS for diagnosing PTB was 83.3 %, which was better than that of smear 11.9 %, culture 40.5 % or Xpert38.1 % (all p < 0.01). The area under the curve (AUC) value of MALDI⁃TOFMS was 0.889, which was better than that of smear 0.532, culture 0.675 or Xpert 0.690 (all p < 0.01). The katG315 and rpoB-RRDR 511 mutations were detected by the MALDI⁃TOFMS in two patients.
UNASSIGNED: Nucleotide MALDI-TOFMS has a good clinical performance for rapid diagnosis of PTB from BALF samples in HIV infected patients, and detects mutations of TB simultaneously.
摘要：
■肺结核（PTB）仍然是一个重大的健康问题，特别是在感染人类免疫缺陷病毒（HIV）的个体中，这些个体更容易发展为活动性TB疾病。结核病的早期和准确诊断对于有效治疗和预防传播至关重要。本研究旨在评估支气管肺泡灌洗液（BALF）的基质辅助激光解吸电离飞行时间质谱（MALDI-TOFMS）分析在HIV感染患者中诊断可疑PTB的潜力。
■这项回顾性研究招募了60名在2022年1月至2023年6月之间出现呼吸道症状和胸部X光片异常的疑似PTB的HIV感染患者。收集BALF样品并使用MALDI-TOFMS进行分析，GeneXpert,抗酸杆菌（AFB）涂片和培养。并比较了它们的诊断性能。
■MALDI测度TOFMS诊断PTB的灵敏度为83.3%,优于涂片11.9%，培养40.5%或Xpert38.1%(所有p<0.01)。MALDI＋TOFMS的曲线下面积（AUC）值为0.889，优于涂片0.532、培养0.675或Xpert0.690（均p<0.01）。在2例患者中通过MALDI-TOFMS检测到katG315和rpoB-RRDR511突变。
■核苷酸MALDI-TOFMS对于HIV感染患者的BALF样本中PTB的快速诊断具有良好的临床性能，同时检测TB的突变。
■这项回顾性研究招募了60名在2022年1月至2023年6月之间出现呼吸道症状和胸部X光片异常的疑似PTB的HIV感染患者。收集BALF样品并使用MALDI-TOFMS进行分析，GeneXpert,抗酸杆菌（AFB）涂片和培养。并比较了它们的诊断性能。
■MALDI测度TOFMS诊断PTB的灵敏度为83.3%,优于涂片11.9%，培养40.5%或Xpert38.1%(所有p<0.01)。MALDI＋TOFMS的曲线下面积（AUC）值为0.889，优于涂片0.532、培养0.675或Xpert0.690（均p<0.01）。在2例患者中通过MALDI-TOFMS检测到katG315和rpoB-RRDR511突变。
■核苷酸MALDI-TOFMS对于HIV感染患者的BALF样本中PTB的快速诊断具有良好的临床性能，同时检测TB的突变。
