UNASSIGNED: This retrospective study recruited 60 HIV-infected patients with suspected PTB presenting with respiratory symptoms and abnormal chest radiographs between January 2022 and June 2023. BALF samples were collected and subjected to analysis using MALDI-TOF MS, GeneXpert, acid-fast bacilli (AFB) smear and culture. And their diagnostic performance was compared.
UNASSIGNED: The sensitivity of MALDI⁃TOFMS for diagnosing PTB was 83.3 %, which was better than that of smear 11.9 %, culture 40.5 % or Xpert38.1 % (all p < 0.01). The area under the curve (AUC) value of MALDI⁃TOFMS was 0.889, which was better than that of smear 0.532, culture 0.675 or Xpert 0.690 (all p < 0.01). The katG315 and rpoB-RRDR 511 mutations were detected by the MALDI⁃TOFMS in two patients.
UNASSIGNED: Nucleotide MALDI-TOFMS has a good clinical performance for rapid diagnosis of PTB from BALF samples in HIV infected patients, and detects mutations of TB simultaneously.
■这项回顾性研究招募了60名在2022年1月至2023年6月之间出现呼吸道症状和胸部X光片异常的疑似PTB的HIV感染患者。收集BALF样品并使用MALDI-TOFMS进行分析,GeneXpert,抗酸杆菌(AFB)涂片和培养。并比较了它们的诊断性能。
■MALDI测度TOFMS诊断PTB的灵敏度为83.3%,优于涂片11.9%,培养40.5%或Xpert38.1%(所有p<0.01)。MALDI+TOFMS的曲线下面积(AUC)值为0.889,优于涂片0.532、培养0.675或Xpert0.690(均p<0.01)。在2例患者中通过MALDI-TOFMS检测到katG315和rpoB-RRDR511突变。
■核苷酸MALDI-TOFMS对于HIV感染患者的BALF样本中PTB的快速诊断具有良好的临床性能,同时检测TB的突变。