UNASSIGNED: Human lenses of various ages were divided into five donor groups: transparent lenses (18-22-year-old, 48-64-year-old, and 70-93-year-old) and cataractous human lenses of two age groups (48-64-year-old lenses, and 70-93-year-old lenses) and were subjected to proteomic analysis. Relative DHA and DHB peptide levels were quantified and compared to their non-modified peptide counterparts.
UNASSIGNED: For most lens proteins containing DHA or DHB, higher amounts of DHA- and DHB-modified peptides were detected in aged and cataractous lenses. DHA-containing peptides were classified into three groups based on abundance changes with age and cataract: those that (1) increased only in age-related nuclear cataract (ARNC), (2) increased in aged and cataractous lenses, and (3) decreased in aged lenses and ARNC. There was no indication that DHA or DHB levels were dependent on lens region. In most donor groups, proteins with DHA and DHB were more likely to be found among urea-insoluble proteins rather than among water- or urea-soluble proteins.
UNASSIGNED: DHA and DHB formation may induce structural effects that make proteins less soluble in water that leads to age-related protein insolubility and possibly aggregation and light scattering.
■将不同年龄的人类镜片分为五个供体组:透明镜片(18-22岁,48-64岁,和70-93岁)和两个年龄组的白内障人类晶状体(48-64岁的晶状体,和70-93岁的镜片),并进行了蛋白质组学分析。定量相对DHA和DHB肽水平并与其未修饰的肽对应物进行比较。
■对于大多数含有DHA或DHB的晶状体蛋白,在老年和白内障晶状体中检测到较高量的DHA和DHB修饰肽。根据年龄和白内障的丰度变化,将含DHA的肽分为三组:(1)仅在年龄相关性核性白内障(ARNC)中增加,(2)老年和白内障晶状体增加,(3)老年晶状体和ARNC下降。没有迹象表明DHA或DHB水平依赖于晶状体区域。在大多数捐赠团体中,含有DHA和DHB的蛋白质更可能存在于尿素不溶性蛋白质中,而不是水溶性或尿素可溶性蛋白质中.
■DHA和DHB的形成可能会引起结构效应,使蛋白质在水中的溶解度降低,从而导致与年龄相关的蛋白质不溶性以及可能的聚集和光散射。