PDF(Pubmed)
Abstract：
Meibomian gland dysfunction (MGD) is a prevalent worldwide eye disorder that causes eye irritation, inflammation, chronic dryness, and blurred vision. Traditional therapies offer temporary improvement, but their efficacy varies in severe MGD cases. Ocular intense pulsed light (IPL) has emerged as a novel therapy, providing long-term symptom relief and shorter treatment durations compared to traditional approaches. However, the impact of IPL on the bacterial community within the eyes remains limited. To address this, we conducted a preliminary study using metagenomics and next-generation sequencing. We compared the bacterial eyelash communities of Thai females with severe MGD before and after 2-4 IPL treatments, and against a group of healthy females. Our findings revealed higher bacterial diversity in healthy individuals compared to severe MGD cases. IPL treatments increased diversity in the MGD group, making their core bacterial community more similar to that of healthy subjects. Notably, the presence of Koribacteraceae in severe MGD and Bifidobacterium in healthy individuals and post-IPL-treated MGD exemplified this shift. Clustering analysis showed a closer relationship between post-IPL-treated MGH and healthy subjects, while the pre-IPL treatment group formed a separate branch. These results suggest that IPL treatment can reshape the eyelash microbiome in MGD cases, but further research is needed to understand the implications and the microbiome\'s role in MGD pathogenesis and treatment response.
摘要：
睑板腺功能障碍（MGD）是一种普遍的全球眼病，可引起眼睛刺激，炎症,慢性干燥，和视力模糊。传统疗法提供暂时的改善，但在严重MGD病例中疗效不同。眼强脉冲光（IPL）已经成为一种新的疗法，与传统方法相比，提供长期症状缓解和较短的治疗持续时间。然而,IPL对眼内细菌群落的影响仍然有限。为了解决这个问题，我们使用宏基因组学和下一代测序进行了初步研究.我们比较了2-4IPL治疗前后泰国女性重度MGD的细菌睫毛群落，和一群健康的女性。我们的发现表明，与严重MGD病例相比，健康个体的细菌多样性更高。IPL治疗增加了MGD组的多样性，使他们的核心细菌群落与健康受试者更相似。值得注意的是,在健康个体的重度MGD和双歧杆菌中以及IPL治疗后的MGD中存在Koribacteraceae，这说明了这种转变。聚类分析显示IPL治疗后MGH与健康受试者之间的关系更密切，而IPL前治疗组形成了一个单独的分支。这些结果表明，IPL治疗可以重塑MGD病例的睫毛微生物组，但需要进一步的研究来了解MGD发病机制和治疗反应中的意义和微生物组的作用。
