PDF(Pubmed)
Abstract：
UNASSIGNED: Blood pressure (BP) regulation is a complex process involving several factors, among which water-sodium balance holds a prominent place. Arginin-vasopressin (AVP), a key player in water metabolism, has been evoked in hypertension development since the 1980s, but, to date, the matter is still controversial. Hyaluronic acid metabolism has been reported to be involved in renal water management, and AVP appears to increase hyaluronidase activity resulting in decreased high-molecular-weight hyaluronan content in the renal interstitium, facilitating water reabsorption in collecting ducts. Hence, our aim was to evaluate urinary hyaluronidase activity in response to an oral water load in hypertensive patients (HT, n=21) compared to normotensive subjects with (NT+, n=36) and without (NT-, n=29) a family history of hypertension, and to study its association with BP and AVP system activation, expressed by serum copeptin levels and urine Aquaporin 2 (AQP2)/creatinine ratio.
UNASSIGNED: Eighty-six Caucasian men were studied. Water load test consisted in oral administration of 15-20 ml of water/kg body weight over 40-45 min. BP, heart rate, serum copeptin, urine hyaluronidase activity and AQP2 were monitored for 4 hours.
UNASSIGNED: In response to water drinking, BP raised in all groups with a peak at 20-40 min. Baseline levels of serum copeptin, urinary hyaluronidase activity and AQP2/creatinine ratio were similar among groups and all decreased after water load, reaching their nadir at 120 min and then gradually recovering to baseline values. Significantly, a blunted reduction in serum copeptin, urinary hyaluronidase activity and AQP2/creatinine ratio was observed in NT+ compared to NT- subjects. A strong positive correlation was also found between urinary hyaluronidase activity and AQP2/creatinine ratio, and, although limited to the NT- group, both parameters were positively associated with systolic BP.
UNASSIGNED: Our results demonstrate for the first time the existence in men of a close association between urinary hyaluronidase activity and vasopressinergic system and suggest that NT+ subjects have a reduced ability to respond to water loading possibly contributing to the blood volume expansion involved in early-stage hypertension. Considering these data, AVP could play a central role in BP regulation by affecting water metabolism through both hyaluronidase activity and AQP2 channel expression.
UNASSIGNED: Eighty-six Caucasian men were studied. Water load test consisted in oral administration of 15-20 ml of water/kg body weight over 40-45 min. BP, heart rate, serum copeptin, urine hyaluronidase activity and AQP2 were monitored for 4 hours.
UNASSIGNED: In response to water drinking, BP raised in all groups with a peak at 20-40 min. Baseline levels of serum copeptin, urinary hyaluronidase activity and AQP2/creatinine ratio were similar among groups and all decreased after water load, reaching their nadir at 120 min and then gradually recovering to baseline values. Significantly, a blunted reduction in serum copeptin, urinary hyaluronidase activity and AQP2/creatinine ratio was observed in NT+ compared to NT- subjects. A strong positive correlation was also found between urinary hyaluronidase activity and AQP2/creatinine ratio, and, although limited to the NT- group, both parameters were positively associated with systolic BP.
UNASSIGNED: Our results demonstrate for the first time the existence in men of a close association between urinary hyaluronidase activity and vasopressinergic system and suggest that NT+ subjects have a reduced ability to respond to water loading possibly contributing to the blood volume expansion involved in early-stage hypertension. Considering these data, AVP could play a central role in BP regulation by affecting water metabolism through both hyaluronidase activity and AQP2 channel expression.
摘要：
■血压（BP）调节是一个复杂的过程，涉及多个因素，其中水钠平衡占有重要地位。精氨酸加压素（AVP），水代谢的关键角色，自20世纪80年代以来一直在高血压的发展中引起，但是,到目前为止,这件事仍然有争议。据报道，透明质酸代谢与肾脏水管理有关，和AVP似乎增加透明质酸酶活性，导致肾间质中高分子量透明质酸含量降低，促进水的重吸收在收集管道。因此，我们的目的是评估高血压患者对口服水负荷的尿透明质酸酶活性（HT，n=21）与血压正常的受试者相比（NT+，n=36)和无(NT-，n=29）有高血压家族史，并研究其与BP和AVP系统激活的关系，以血清和肽素水平和尿液水通道蛋白2（AQP2）/肌酐比值表示。
■研究了86名白人男性。水负荷试验包括在40-45分钟内口服15-20ml水/kg体重。BP,心率,血清和肽素，监测尿透明质酸酶活性和AQP24小时.
■为了应对饮用水，所有组的BP均在20-40分钟达到峰值。血清和肽素的基线水平，尿透明质酸酶活性和AQP2/肌酐比值在各组之间相似，并且在水负荷后均下降，在120分钟达到最低点，然后逐渐恢复到基线值。重要的是,血清和肽素的减少，与NT-受试者相比,在NT+受试者中观察到尿透明质酸酶活性和AQP2/肌酐比值.尿透明质酸酶活性与AQP2/肌酐比值之间也有很强的正相关，and,虽然仅限于NT-组，这两个参数均与收缩压呈正相关.
■我们的结果首次证明了男性尿透明质酸酶活性与血管加压素能系统之间存在密切联系，并表明NT受试者对水负荷的反应能力降低可能导致早期高血压患者的血容量膨胀。考虑到这些数据,AVP可以通过透明质酸酶活性和AQP2通道表达影响水代谢，在BP调节中发挥核心作用。
■研究了86名白人男性。水负荷试验包括在40-45分钟内口服15-20ml水/kg体重。BP,心率,血清和肽素，监测尿透明质酸酶活性和AQP24小时.
■为了应对饮用水，所有组的BP均在20-40分钟达到峰值。血清和肽素的基线水平，尿透明质酸酶活性和AQP2/肌酐比值在各组之间相似，并且在水负荷后均下降，在120分钟达到最低点，然后逐渐恢复到基线值。重要的是,血清和肽素的减少，与NT-受试者相比,在NT+受试者中观察到尿透明质酸酶活性和AQP2/肌酐比值.尿透明质酸酶活性与AQP2/肌酐比值之间也有很强的正相关，and,虽然仅限于NT-组，这两个参数均与收缩压呈正相关.
■我们的结果首次证明了男性尿透明质酸酶活性与血管加压素能系统之间存在密切联系，并表明NT受试者对水负荷的反应能力降低可能导致早期高血压患者的血容量膨胀。考虑到这些数据,AVP可以通过透明质酸酶活性和AQP2通道表达影响水代谢，在BP调节中发挥核心作用。
