RESULTS: We identified the fall webworm gustatory receptor HcGr76 as a fructose and chlorogenic acid receptor using Ca2+ imaging and determination of intracellular Ca2+ concentration by a microplate reader. Moreover, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis revealed that HcGr76 is highly expressed in the anterior and middle midgut. Knockdown of HcGr76 caused a significant reduction in the expression of neuropeptide F 1 (NPF1) and CCHamide-2, and led to a decrease in carbohydrate and lipid levels in the hemolymph.
CONCLUSIONS: Our studies provide circumstantial evidence that HcGr76 expressed in the midgut is involved in sensing dietary components, and regulates the expression of relevant peptide hormones to alter metabolism in H. cunea larvae, thus providing a promising molecular target for the development of new insect-specific control products. © 2024 Society of Chemical Industry.
结果:我们使用Ca2+成像和通过酶标仪测定细胞内Ca2+浓度,鉴定了秋季网虫味觉受体HcGr76为果糖和绿原酸受体。此外,逆转录-定量聚合酶链反应(RT-qPCR)分析显示HcGr76在前肠和中肠高表达。HcGr76的敲低导致神经肽F1(NPF1)和CCHamide-2的表达显着降低,并导致血淋巴中碳水化合物和脂质水平降低。
结论:我们的研究提供了间接证据,表明中肠中表达的HcGr76与感知饮食成分有关,并调节相关肽激素的表达,以改变库纳幼虫的代谢,从而为开发新的昆虫特异性防治产品提供了有希望的分子靶标。©2024化学工业学会。