Abstract：
BACKGROUND: Sensing dietary components in the gut is important to ensure an appropriate hormonal response and metabolic regulation after food intake. The fall webworm, Hyphantria cunea, is a major invasive pest in China and has led to significant economic losses and ecosystem disruption. The larvae\'s broad host range and voracious appetite for leaves make H. cunea a primary cause of serious damage to both forests and crops. To date, however, the gustatory receptors (Grs) of H. cunea and their regulatory function remain largely unknown.
RESULTS: We identified the fall webworm gustatory receptor HcGr76 as a fructose and chlorogenic acid receptor using Ca2+ imaging and determination of intracellular Ca2+ concentration by a microplate reader. Moreover, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis revealed that HcGr76 is highly expressed in the anterior and middle midgut. Knockdown of HcGr76 caused a significant reduction in the expression of neuropeptide F 1 (NPF1) and CCHamide-2, and led to a decrease in carbohydrate and lipid levels in the hemolymph.
CONCLUSIONS: Our studies provide circumstantial evidence that HcGr76 expressed in the midgut is involved in sensing dietary components, and regulates the expression of relevant peptide hormones to alter metabolism in H. cunea larvae, thus providing a promising molecular target for the development of new insect-specific control products. © 2024 Society of Chemical Industry.
RESULTS: We identified the fall webworm gustatory receptor HcGr76 as a fructose and chlorogenic acid receptor using Ca2+ imaging and determination of intracellular Ca2+ concentration by a microplate reader. Moreover, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis revealed that HcGr76 is highly expressed in the anterior and middle midgut. Knockdown of HcGr76 caused a significant reduction in the expression of neuropeptide F 1 (NPF1) and CCHamide-2, and led to a decrease in carbohydrate and lipid levels in the hemolymph.
CONCLUSIONS: Our studies provide circumstantial evidence that HcGr76 expressed in the midgut is involved in sensing dietary components, and regulates the expression of relevant peptide hormones to alter metabolism in H. cunea larvae, thus providing a promising molecular target for the development of new insect-specific control products. © 2024 Society of Chemical Industry.
摘要：
背景：感测肠道中的饮食成分对于确保食物摄入后适当的激素反应和代谢调节很重要。秋天的网虫，美国白蛾,是中国主要的入侵害虫，并已导致重大的经济损失和生态系统破坏。幼虫广泛的寄主范围和对叶子的贪婪食欲使H.cunea成为严重损害森林和农作物的主要原因。迄今为止,然而,H.cunea的味觉受体（Grs）及其调节功能在很大程度上仍然未知。
结果：我们使用Ca2+成像和通过酶标仪测定细胞内Ca2+浓度，鉴定了秋季网虫味觉受体HcGr76为果糖和绿原酸受体。此外，逆转录-定量聚合酶链反应(RT-qPCR)分析显示HcGr76在前肠和中肠高表达。HcGr76的敲低导致神经肽F1（NPF1）和CCHamide-2的表达显着降低，并导致血淋巴中碳水化合物和脂质水平降低。
结论：我们的研究提供了间接证据，表明中肠中表达的HcGr76与感知饮食成分有关，并调节相关肽激素的表达，以改变库纳幼虫的代谢，从而为开发新的昆虫特异性防治产品提供了有希望的分子靶标。©2024化学工业学会。
结果：我们使用Ca2+成像和通过酶标仪测定细胞内Ca2+浓度，鉴定了秋季网虫味觉受体HcGr76为果糖和绿原酸受体。此外，逆转录-定量聚合酶链反应(RT-qPCR)分析显示HcGr76在前肠和中肠高表达。HcGr76的敲低导致神经肽F1（NPF1）和CCHamide-2的表达显着降低，并导致血淋巴中碳水化合物和脂质水平降低。
结论：我们的研究提供了间接证据，表明中肠中表达的HcGr76与感知饮食成分有关，并调节相关肽激素的表达，以改变库纳幼虫的代谢，从而为开发新的昆虫特异性防治产品提供了有希望的分子靶标。©2024化学工业学会。
