PDF(Pubmed)
Abstract：
Pre-mRNA splicing is a significant step for post-transcriptional modifications and functions in a wide range of physiological processes in plants. Human NHP2L binds to U4 snRNA during spliceosome assembly; it is involved in RNA splicing and mediates the development of human tumors. However, no ortholog has yet been identified in plants. Therefore, we report At4g12600 encoding the ortholog NHP2L protein, and AtSNU13 associates with the component of the spliceosome complex; the atsnu13 mutant showed compromised resistance in disease resistance, indicating that AtSNU13 is a positive regulator of plant immunity. Compared to wild-type plants, the atsnu13 mutation resulted in altered splicing patterns for defense-related genes and decreased expression of defense-related genes, such as RBOHD and ALD1. Further investigation shows that AtSNU13 promotes the interaction between U4/U6.U5 tri-snRNP-specific 27 K and the motif in target mRNAs to regulate the RNA splicing. Our study highlights the role of AtSNU13 in regulating plant immunity by affecting the pre-mRNA splicing of defense-related genes.
摘要：
前mRNA剪接是转录后修饰的重要步骤，并在植物的各种生理过程中发挥作用。人NHP2L在剪接体组装过程中与U4snRNA结合;它参与RNA剪接并介导人类肿瘤的发展。然而,在植物中还没有发现直系同源物。因此,我们报道了At4g12600编码直系同源NHP2L蛋白，和AtSNU13与剪接体复合物的成分相关；atsnu13突变体在抗病性方面表现出受损的抗性，表明AtSNU13是植物免疫的正调节因子。与野生型植物相比，atsnu13突变导致防御相关基因的剪接模式改变和防御相关基因的表达减少，例如RBOHD和ALD1。进一步研究表明，AtSNU13促进U4/U6之间的相互作用。U5三-snRNP特异性27K和目标mRNA中的基序调节RNA剪接。我们的研究强调了AtSNU13通过影响防御相关基因的前mRNA剪接在调节植物免疫中的作用。
