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Abstract：
BACKGROUND: Protein phosphatase class 2 C (PP2C) is the largest protein phosphatase family in plants. Members of the PP2C gene family are involved in a variety of physiological pathways in plants, including the abscisic acid signalling pathway, the regulation of plant growth and development, etc., and are capable of responding to a wide range of biotic and abiotic stresses, and play an important role in plant growth, development, and response to stress. Apocynum is a perennial persistent herb, divided into Apocynum venetum and Apocynum hendersonii. It mainly grows in saline soil, deserts and other harsh environments, and is widely used in saline soil improvement, ecological restoration, textiles and medicine. A. hendersonii was found to be more tolerant to adverse conditions. The main purpose of this study was to investigate the PP2C gene family and its expression pattern under salt stress and to identify important candidate genes related to salt tolerance.
RESULTS: In this study, 68 AvPP2C genes and 68 AhPP2C genes were identified from the genomes of A. venetum and A. hendersonii, respectively. They were classified into 13 subgroups based on their phylogenetic relationships and were further analyzed for their subcellular locations, gene structures, conserved structural domains, and cis-acting elements. The results of qRT-PCR analyses of seven AvPP2C genes and seven AhPP2C genes proved that they differed significantly in gene expression under salt stress. It has been observed that the PP2C genes in A. venetum and A. hendersonii exhibit different expression patterns. Specifically, AvPP2C2, 6, 24, 27, 41 and AhPP2C2, 6, 24, 27, 42 have shown significant differences in expression under salt stress. This indicates that these genes may play a crucial role in the salt tolerance mechanism of A. venetum and A. hendersonii.
CONCLUSIONS: In this study, we conducted a genome-wide analysis of the AvPP2C and AhPP2C gene families in Apocynum, which provided a reference for further understanding the functional characteristics of these genes.
RESULTS: In this study, 68 AvPP2C genes and 68 AhPP2C genes were identified from the genomes of A. venetum and A. hendersonii, respectively. They were classified into 13 subgroups based on their phylogenetic relationships and were further analyzed for their subcellular locations, gene structures, conserved structural domains, and cis-acting elements. The results of qRT-PCR analyses of seven AvPP2C genes and seven AhPP2C genes proved that they differed significantly in gene expression under salt stress. It has been observed that the PP2C genes in A. venetum and A. hendersonii exhibit different expression patterns. Specifically, AvPP2C2, 6, 24, 27, 41 and AhPP2C2, 6, 24, 27, 42 have shown significant differences in expression under salt stress. This indicates that these genes may play a crucial role in the salt tolerance mechanism of A. venetum and A. hendersonii.
CONCLUSIONS: In this study, we conducted a genome-wide analysis of the AvPP2C and AhPP2C gene families in Apocynum, which provided a reference for further understanding the functional characteristics of these genes.
摘要：
背景：蛋白磷酸酶2C类（PP2C）是植物中最大的蛋白磷酸酶家族。PP2C基因家族成员参与植物的多种生理通路,包括脱落酸信号通路,植物生长和发育的调节，等。,并且能够应对广泛的生物和非生物胁迫，并在植物生长中发挥重要作用，发展,和对压力的反应。罗布麻是一种多年生的持久性草本植物，分为罗布麻和罗布麻。它主要生长在盐渍土壤中，沙漠和其他恶劣环境，广泛用于盐渍土改良，生态恢复,纺织品和医药。发现隐球菌对不利条件的耐受性更强。本研究的主要目的是研究盐胁迫下PP2C基因家族及其表达模式，并鉴定与耐盐相关的重要候选基因。
结果：在这项研究中，从A.venetum和A.hendersonii的基因组中鉴定出68个AvPP2C基因和68个AhPP2C基因，分别。根据系统发育关系将它们分为13个亚组，并进一步分析了它们的亚细胞位置，基因结构，保守的结构域，和顺式行动元素。对7个AvPP2C基因和7个AhPP2C基因的qRT-PCR分析结果证明，在盐胁迫下，它们在基因表达方面存在显着差异。已经观察到,在A.venetum和A.hendersonii中的PP2C基因表现出不同的表达模式。具体来说,AvPP2C2、6、24、27、41和AhPP2C2、6、24、27、42在盐胁迫下的表达均显示出显著差异。这表明这些基因可能在A.venetum和A.hendersonii的耐盐机制中起着至关重要的作用。
结论：在这项研究中，我们对罗布麻的AvPP2C和AhPP2C基因家族进行了全基因组分析，为进一步了解这些基因的功能特性提供了参考。
结果：在这项研究中，从A.venetum和A.hendersonii的基因组中鉴定出68个AvPP2C基因和68个AhPP2C基因，分别。根据系统发育关系将它们分为13个亚组，并进一步分析了它们的亚细胞位置，基因结构，保守的结构域，和顺式行动元素。对7个AvPP2C基因和7个AhPP2C基因的qRT-PCR分析结果证明，在盐胁迫下，它们在基因表达方面存在显着差异。已经观察到,在A.venetum和A.hendersonii中的PP2C基因表现出不同的表达模式。具体来说,AvPP2C2、6、24、27、41和AhPP2C2、6、24、27、42在盐胁迫下的表达均显示出显著差异。这表明这些基因可能在A.venetum和A.hendersonii的耐盐机制中起着至关重要的作用。
结论：在这项研究中，我们对罗布麻的AvPP2C和AhPP2C基因家族进行了全基因组分析，为进一步了解这些基因的功能特性提供了参考。
