PDF(Pubmed)
Abstract：
45S5 Bioglass (BG) is composed of a glass network with silicate based on the component and can be doped with various therapeutic ions for the enhancement of hard tissue therapy. Nanoceria (CeO2) has been shown to indicate redox reaction and enhance the biological response. However, few studies focus on the proportion of CeO2-doped and its effect on the cellular bioactivity of CeO2-doped BG (CBG). In this study, we synthesized the CBG series with increasing amounts of doping CeO2 ranging (1 to 12) wt.%. The synthesized CBG series examined the characterization, mineralization capacity, and cellular activity against BG. Our results showed that the CBG series exhibited a glass structure and indicated the redox states between Ce3+ and Ce4+, thus they showed the antioxidant activity by characterization of Ce. The CBG series had a stable glass network structure similar to BG, which showed the preservation of bioactivity by exhibiting mineralization on the surface. In terms of biological response, although the CBG series showed the proliferative activity of pre-osteoblastic cells similar to BG, the CBG series augmented not only the alkaline phosphatase activity but also the osteogenic marker in the mRNA level. As stimulated the osteogenic activity, the CBG series improved the biomineralization. In conclusion, the CBG series might have a potential application for hard tissue therapeutic purposes.
摘要：
45S5Bioglass（BG）由基于组分的硅酸盐的玻璃网络组成，可以掺杂各种治疗离子，用于增强硬组织治疗。已显示纳米二氧化铈(CeO2)指示氧化还原反应并增强生物响应。然而,很少有研究集中在CeO2掺杂的比例及其对CeO2掺杂的BG（CBG）的细胞生物活性的影响。在这项研究中,我们合成了CBG系列，掺杂CeO2的量增加（1至12）wt。%.合成的CBG系列检查了表征，矿化能力，和针对BG的细胞活性。我们的结果表明，CBG系列表现出玻璃结构，并表明Ce3和Ce4之间的氧化还原态，因此，它们通过表征Ce表现出抗氧化活性。CBG系列具有与BG相似的稳定玻璃网络结构，通过在表面表现出矿化作用，显示了生物活性的保存。就生物学反应而言，虽然CBG系列显示前成骨细胞的增殖活性类似于BG，CBG系列不仅增强了碱性磷酸酶活性，而且还增强了mRNA水平的成骨标志物。刺激成骨活性，CBG系列改善了生物矿化。总之,CBG系列可能具有用于硬组织治疗目的的潜在应用。
