PDF(Pubmed)
Abstract：
Infertility, a worldwide reproductive health concern, impacts approximately one in five couples. Male infertility, stemming from spermatogenic dysfunction and reduced sperm quality, stands as a primary factor contributing to infertility. Given the global decrease in male fertility linked to environmental factors like the greenhouse effect, it is crucial to develop a comprehensive understanding of how increased temperatures impact both the quantity and quality of sperm. In this study, we utilized Pandora-seq technology to detect the small non-coding RNAs (sncRNAs) expression profile in the testicular tissue of heat-stressed mice. The investigation explores the dynamic shifts in sncRNAs within the mouse testis under heat stress, including miRNAs, tsRNAs, piRNAs, rsRNAs and other sncRNAs. Furthermore, we successfully identified differentially expressed sncRNAs in testicular tissues before and after heat stress. Subsequently, we conducted functional enrichment analysis on the potential predicted target genes of differentially expressed miRNAs and tsRNAs. These datasets will constitute a valuable foundational resource for further investigations into the decline in male reproductive capacity triggered by heat stress.
摘要：
不孕症,一个世界性的生殖健康问题，影响大约五分之一的夫妇。男性不育,源于生精功能障碍和精子质量下降，是导致不孕症的主要因素。鉴于全球男性生育率下降与温室效应等环境因素有关，全面了解温度升高如何影响精子的数量和质量至关重要。在这项研究中,我们利用Pandora-seq技术检测了热应激小鼠睾丸组织中的非编码小RNA(sncRNAs)表达谱.这项研究探索了热应激下小鼠睾丸中sncRNAs的动态变化，包括miRNAs,tsRNAs,piRNAs,rsRNA和其他sncRNA。此外，我们成功地鉴定了热应激前后睾丸组织中差异表达的sncRNAs。随后，我们对差异表达的miRNAs和tsRNAs的潜在预测靶基因进行了功能富集分析。这些数据集将为进一步研究热应激引发的男性生殖能力下降提供宝贵的基础资源。
