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Abstract：
It is well established that the medial prefrontal cortex (mPFC) exerts top-down control of many behaviors, but little is known regarding how cross-talk between distinct areas of the mPFC influences top-down signaling. We performed virus-mediated tracing and functional studies in male mice, homing in on GABAergic projections whose axons are located mainly in layer 1 and that connect two areas of the mPFC, namely the prelimbic area (PrL) with the cingulate area 1 and 2 (Cg1/2). We revealed the identity of the targeted neurons that comprise two distinct types of layer 1 GABAergic interneurons, namely single-bouquet cells (SBCs) and neurogliaform cells (NGFs), and propose that this connectivity links GABAergic projection neurons with cortical canonical circuits. In vitro electrophysiological and in vivo calcium imaging studies support the notion that the GABAergic projection neurons from the PrL to the Cg1/2 exert a crucial role in regulating the activity in the target area by disinhibiting layer 5 output neurons. Finally, we demonstrated that recruitment of these projections affects impulsivity and mechanical responsiveness, behaviors which are known to be modulated by Cg1/2 activity.
摘要：
众所周知，内侧前额叶皮层（mPFC）对许多行为进行自上而下的控制，但是关于mPFC的不同区域之间的串扰如何影响自顶向下的信令知之甚少。我们在雄性小鼠中进行了病毒介导的追踪和功能研究，归巢于GABA能投影，其轴突主要位于第1层，并连接mPFC的两个区域，即带带区域1和2（Cg1/2）的边缘前区域（PrL）。我们揭示了包含两种不同类型的第1层GABA能中间神经元的靶向神经元的身份，即单束细胞（SBC）和神经胶质细胞（NGF），并提出这种连通性将GABA能投射神经元与皮质规范回路联系起来。体外电生理和体内钙成像研究支持以下观点：从PrL到Cg1/2的GABA能投射神经元通过抑制5层输出神经元在调节目标区域的活动中发挥关键作用。最后,我们证明了这些预测的招募会影响冲动性和机械响应性，已知由Cg1/2活性调节的行为。
