Abstract：
The Malus sylvestris L. (LE1), Pinus sylvestris L. (LE2), and Sorbus aucuparia L. (LE3) leaves` extracts were used for the synthesis of silver (Ag) nanocomposites containing different amounts of silver chloride (AgCl), silver metal (Agmet), and silver phosphate (Ag3PO4). These nanocomposites were capped with the organic functional groups in the leaf extract. Notably, the nanocomposites caused biphasic cytotoxic response on cells; first attributed to the inhibition of cell growth and second to cell death. The nanocomposites were biocompatible with normal embryonic kidney (HEK293) cells in the cytotoxic range for cancer cells. [25(±1) °C synthesis] nanocomposites exhibited the highest cytotoxicity towards HeLa (lethal concentration- LC50 value of 11.4 μg mL-1) and A549 (LC50 value of 14.7 μg mL-1) after 24-h incubation and its efficiency was shown also for the more resistant MCF-7 and MDA-MB-231, however, their respective LC50 values were larger. For the HeLa cell line, this designed nanocomposite exhibited an LC50 value similar to the effective concentration (EC50) value of Cisplatin and about 3 times larger than Doxorubicin. nanocomposite contained Ag3PO4 in the composite and P on the surface, higher AgCl content, smaller crystallite size of all nanoparticle phases, and carbon-rich oxygen-deficient surface compared to all other nanocomposites.
摘要：
樟子树（LE1），樟子松（LE2），和SorbusaucupariaL.（LE3）叶子的提取物用于合成含有不同量氯化银（AgCl）的银（Ag）纳米复合材料，银金属（Agmet），和磷酸银(Ag3PO4)。这些纳米复合材料被叶提取物中的有机官能团覆盖。值得注意的是,纳米复合材料在细胞上引起双相细胞毒性反应;首先归因于细胞生长的抑制，其次归因于细胞死亡。纳米复合材料与正常胚胎肾(HEK293)细胞在癌细胞的细胞毒性范围内是生物相容的。LE2Ag1[25（±1）°C合成]纳米复合材料在24小时孵育后对HeLa（致死浓度-LC50值为11.4μgmL-1）和A549（LC50值为14.7μgmL-1）表现出最高的细胞毒性。他们各自的LC50值较大。对于HeLa细胞系，这种设计的LE2Ag1纳米复合材料的LC50值与顺铂的有效浓度(EC50)值相似,是阿霉素的3倍.LE2Ag1纳米复合材料在复合材料中含有Ag3PO4，表面含有P，较高的AgCl含量，所有纳米粒子相的微晶尺寸较小，与所有其他纳米复合材料相比，富碳缺氧表面。
