PDF(Pubmed)
Abstract：
Cadmium (Cd), is a highly toxic environmental pollutant, which seriously threatens the health of poultry and humans. The occurrence of osteoporosis is the main manifestation of cadmium toxicity. Pyroptosis plays an important role in the development of osteoporosis. Melatonin has been shown to affect preserving bone health. However, the underlying mechanism has not been elucidated. In the present study, these functions of melatonin have been investigated in duck bone tissue and osteoblast during cadmium exposure. In vivo, the studies suggest that melatonin protects against cadmium-induced duck osteoporosis by improving the osteogenesis function, inhibiting bone resorption, and suppressing the occurrence of pyroptosis. In vitro, the findings demonstrated that melatonin alleviated the inhibition effect of cadmium on duck bone marrow-derived mesenchymal stem cells (BMSC) osteogenic differentiation, and suppressed the cadmium-induced osteoclast differentiation. In addition, we also found that melatonin prevents cytokines release of lactate dehydrogenase (LDH), interleukin-18 (IL-18), and interleukin-1β (IL-1β) by cadmium-induced, and reduces the expression of n-terminal Gasdermin D (N-GSDMD), alleviates the osteoblast death rate. In short, melatonin as a potential therapeutic agent has bright prospects in cadmium-induced bone toxicity.
摘要：
镉(Cd),是一种剧毒的环境污染物，严重威胁家禽和人类的健康。骨质疏松症的发生是镉中毒的主要表现。焦亡在骨质疏松的发生发展中起重要作用。褪黑激素已被证明影响保持骨骼健康。然而,潜在的机制尚未阐明。在本研究中,褪黑素的这些功能已在镉暴露期间在鸭骨组织和成骨细胞中进行了研究。在体内,研究表明，褪黑素通过改善成骨功能来预防镉诱导的鸭骨质疏松症，抑制骨吸收，抑制焦亡的发生。体外，结果表明,褪黑素能减轻镉对鸭骨髓间充质干细胞(BMSC)成骨分化的抑制作用,并抑制镉诱导的破骨细胞分化。此外,我们还发现褪黑激素可以防止细胞因子释放乳酸脱氢酶（LDH），白细胞介素-18（IL-18），和镉诱导的白细胞介素-1β（IL-1β），并降低n端GasderminD（N-GSDMD）的表达，降低成骨细胞死亡率。总之,褪黑素作为一种潜在的治疗药物在镉致骨毒性方面具有广阔的前景。
