Abstract：
The aim of this study was to investigate the signaling pathways involved in the proliferation and differentiation of pig Sertoli cells (SCs) mediated by thyroid hormone (T3) to provide a theoretical and practical basis for enhancing pig semen production. The effects of different concentrations of T3 on the proliferation of pig SCs were evaluated using the CCK8 assay. The impact of T3 on the proliferation and differentiation of pig SCs was further examined using RNA-seq, qPCR, and Western Blotting techniques. Additionally, the involvement of the p38 MAPK and NFκB pathways in mediating the effects of T3 on SCs proliferation and differentiation was investigated. Our findings revealed a strong correlation between the dosage of T3 and the inhibition of pig SCs proliferation and promotion of maturation. T3 regulated the activation state of the NFκB signaling pathway by upregulating IKKα, downregulating IKKβ, and promoting IκB phosphorylation. Furthermore, T3 facilitated SCs maturation by upregulating AR and FSHR expression while downregulating KRT-18. In conclusion, T3 inhibits pig SCs proliferation and promote pig SCs maturation through the IKK/NFκB and p38 MAPK pathways. These findings provide valuable insights into the mechanisms by which T3 influences the proliferation and maturation of pig SCs.
摘要：
本研究旨在探讨甲状腺激素(T3)介导的猪支持细胞(SCs)增殖分化过程中的信号通路,为提高猪精液产量提供理论和实践依据。使用CCK8测定评价了不同浓度的T3对猪SC增殖的影响。使用RNA-seq进一步检查T3对猪SCs增殖和分化的影响，qPCR,和西方印迹技术。此外,研究了p38MAPK和NFκB通路介导T3对SCs增殖和分化的影响。我们的发现表明，T3的剂量与抑制猪SCs增殖和促进成熟之间存在很强的相关性。T3通过上调IKKα调节NFκB信号通路的激活状态,下调IKKβ，促进IκB磷酸化。此外，T3通过上调AR和FSHR表达同时下调KRT-18促进SCs成熟。总之,T3通过IKK/NFκB和p38MAPK通路抑制猪SCs增殖并促进猪SCs成熟。这些发现为T3影响猪SC增殖和成熟的机制提供了有价值的见解。
