Abstract：
With the successful control of rabies transmitted by dogs in Brazil, wild animals have played a relevant epidemiological role in the transmission of rabies virus (RABV). Bats, non-human primates and wild canines are the main wild animals that transmit RABV in the country. It is worth highlighting the possibility of synanthropic action of these species, when they become adapted to urban areas, causing infections in domestic animals and eventually in humans. This work aimed to evaluate the circulation of RABV in the Pedra Branca Forest, an Atlantic Forest area, located in the state of Rio de Janeiro, Southeast Brazil. Saliva and blood samples were obtained from 60 individuals of eight species of bats, captured with mist nets, and 13 individuals of callitrichid primates, captured with tomahawk traps. Saliva samples were subjected to Reverse Transcription Polymerase Chain Reaction (RT-PCR), targeting the RABV N gene, with all samples being negative. Blood samples of all animals were submitted to the Rapid Fluorescent Focus Inhibition Test (RFFIT) to detect neutralizing antibodies (Ab) for RABV. Six bat samples (8%) were seropositive for RABV with antibody titers greater than or equal to 0.1 IU/mL. The detection of Ab but not viral RNA indicates exposure rather than current RABV transmission in the analyzed populations. The results presented here reinforce the importance of serological studies in wildlife to access RABV circulation in a region.
摘要：
随着巴西成功控制了狗传播的狂犬病，野生动物在狂犬病病毒（RABV）的传播中发挥了相关的流行病学作用。蝙蝠,非人灵长类动物和野生犬科动物是该国传播RABV的主要野生动物。值得强调的是这些物种同人化作用的可能性，当它们适应城市地区时，导致家畜感染，最终导致人类感染。这项工作旨在评估佩德拉布兰卡森林中RABV的循环，大西洋森林地区,位于里约热内卢州，巴西东南部。从八种蝙蝠的60个个体中获取唾液和血液样本，用薄雾网捕获，和13个愈伤组织灵长类动物个体，用战斧陷阱捕获.唾液样品进行逆转录聚合酶链反应（RT-PCR），靶向RABVN基因，所有样本都是阴性的。将所有动物的血液样品进行快速荧光聚焦抑制测试(RFFIT)以检测RABV的中和抗体(Ab)。六个蝙蝠样品（8％）对RABV呈血清阳性，抗体滴度大于或等于0.1IU/mL。Ab而不是病毒RNA的检测表明暴露而不是分析群体中的当前RABV传播。此处显示的结果加强了在野生动植物中进行血清学研究以获取该地区RABV循环的重要性。
