Abstract：
The P-type ATPase superfamily genes are the cation and phospholipid pumps that transport ions across the membranes by hydrolyzing ATP. They are involved in a diverse range of functions, including fundamental cellular events that occur during the growth of plants, especially in the reproductive organs. The present work has been undertaken to understand and characterize the P-type ATPases in the pigeonpea genome and their potential role in anther development and pollen fertility. A total of 59 P-type ATPases were predicted in the pigeonpea genome. The phylogenetic analysis classified the ATPases into five subfamilies: eleven P1B, eighteen P2A/B, fourteen P3A, fifteen P4, and one P5. Twenty-three pairs of P-type ATPases were tandemly duplicated, resulting in their expansion in the pigeonpea genome during evolution. The orthologs of the reported anther development-related genes were searched in the pigeonpea genome, and the expression profiling studies of specific genes via qRT-PCR in the pre- and post-meiotic anther stages of AKCMS11A (male sterile), AKCMS11B (maintainer) and AKPR303 (fertility restorer) lines of pigeonpea was done. Compared to the restorer and maintainer lines, the down-regulation of CcP-typeATPase22 in the post-meiotic anthers of the male sterile line might have played a role in pollen sterility. Furthermore, the strong expression of CcP-typeATPase2 in the post-meiotic anthers of restorer line and CcP-typeATPase46, CcP-typeATPase51, and CcP-typeATPase52 in the maintainer lines, respectively, compared to the male sterile line, clearly indicates their potential role in developing male reproductive organs in pigeonpea.
摘要：
P型ATP酶超家族基因是阳离子和磷脂泵,其通过水解ATP转运离子穿过膜。它们涉及各种各样的功能，包括植物生长过程中发生的基本细胞事件，尤其是生殖器官。已经进行了当前工作，以了解和表征木豆基因组中的P型ATPases及其在花药发育和花粉育性中的潜在作用。在木豆基因组中预测了总共59个P型ATPase。系统发育分析将ATPases分为五个亚家族：11个P1B，18P2A/B,14个P3A,15个P4和1个P5.23对P型ATP酶被重复，导致它们在进化过程中在木豆基因组中扩增。在木豆基因组中搜索了已报道的花药发育相关基因的直系同源物，以及通过qRT-PCR对AKCMS11A（雄性不育）减数分裂前和减数分裂后花药阶段中特定基因的表达谱研究，对木豆的AKCMS11B（保持系）和AKPR303（育性恢复系）进行了研究。与修复者和维护者相比，雄性不育系减数分裂后花药中CcP-typeATPase22的下调可能在花粉不育中起作用。此外，CcP-typeATPase2在恢复系减数分裂后花药中的强表达，CcP-typeATPase46，CcP-typeATPase51和CcP-typeATPase52在保持系中的强表达，分别,与雄性不育系相比，清楚地表明了它们在木豆雄性生殖器官发育中的潜在作用。
