Abstract：
Pollutant biomonitoring demands analytical methods to cover a wide range of target compounds, work with minimal sample amounts, and apply least invasive and reproducible sampling procedures. We developed a method to analyse 68 bioaccumulative organic pollutants in three seabird matrices: plasma, liver, and stomach oil, representing different exposure phases. Extraction efficiency was assessed based on recoveries of spiked surrogate samples, then the method was applied to environmental samples collected from Scopoli\'s shearwater (Calonectris diomedea). Extraction was performed in an ultrasonic bath, purification with Florisil cartridges (5 g, 20 mL), and analysis by GC-Orbitrap-MS. Quality controls at 5 ng yielded satisfactory recoveries (80-120%) although signal intensification was found for some compounds. The method permitted the detection of 28 targeted pollutants in the environmental samples. The mean sum of organic pollutants was 4.25 ± 4.83 ng/g in plasma, 1634 ± 2990 ng/g in liver, and 233 ± 111 ng/g in stomach oil (all wet weight). Pollutant profiles varied among the matrices, although 4,4\'-DDE was the dominant compound overall. This method is useful for pollutant biomonitoring in seabirds and discusses the interest of analysing different matrices.
摘要：
污染物生物监测需要分析方法来涵盖广泛的目标化合物，以最少的样本量工作，并应用最小侵入性和可重复的采样程序。我们开发了一种方法来分析三种海鸟基质中的68种生物累积有机污染物：等离子体，肝脏,和胃油，代表不同的曝光阶段。根据加标替代样品的回收率评估提取效率，然后将该方法应用于从Scopoli\'sshearwater（Calonectrisdiomedea）收集的环境样品。在超声波浴中进行提取，用Florisil药筒纯化(5g，20mL),并通过GC-Orbitrap-MS进行分析。5ng的质量控制产生了令人满意的回收率（80-120％），尽管发现某些化合物的信号增强。该方法允许检测环境样品中的28种目标污染物。血浆中有机污染物的平均总和为4.25±4.83ng/g，肝脏中1634±2990ng/g，和233±111ng/g的胃油（所有湿重）。污染物分布在矩阵之间变化，虽然4,4'-DDE是总体上占主导地位的化合物。此方法可用于海鸟中的污染物生物监测，并讨论了分析不同基质的兴趣。
