PDF(Pubmed)
Abstract：
Esophageal cancer (EC) is a multifaceted disease. Our understanding of the involvement of esophageal microbiota in its pathogenesis and progression is limited, which is due to the lack of proper endoscopic sampling methods. Hereby, we conducted a comparative analysis of paired samples obtained through endoscopic brushing and cytosponge, aiming at assessing the feasibility of using cytosponge as a minimally invasive sampling way for studying esophageal microbiota. Our findings suggest that cytosponge sampling yielded significantly superior community richness and diversity compared to endoscopic brushing in both controls (non-cancerous) and EC individuals. The analysis of beta-diversity revealed distinct microbial community pattern in the genus diversity between the two sampling methods, underscoring the importance of selecting appropriate sampling methods to effectively characterize the esophageal microbiota. Specifically, Lactococcus and Serratia showed higher abundance in the samples collected by endoscopic brushing, while Alloprevotella and Leptotrichia were more enriched in the samples collected by cytosponge. These differences in dominant microbes were associated with metabolic pathways that particularly were related to host inflammation, such as pyruvate and glucose metabolisms. Notably, the phylogenetic levels of the microbiota indicated varied explanatory power for different detection purposes. This study underscores the substantial impact of sampling method selection on the acquisition of esophageal microbiota associated with the EC development, encompassing considerations of both abundance and diversity. This highlights the significance of selecting an appropriate sampling method for investigating the esophageal microbial status and studying the micro-environment in EC-related individuals.
OBJECTIVE: This study addresses a critical issue in esophageal cancer study by comparing two different sampling methods, endoscopic brushing and cytosponge, for investigating the esophageal microbiota. Our work highlights the suitability of the cytosponge technique as a minimally invasive sampling method for studying the esophageal microbiota and emphasizes the importance of selecting an appropriate sampling method to characterize the microbial community. Our findings have significant implications for advancing the understanding of the role of the esophageal microbiota in cancer development and will inform future research and clinical approaches in this field.
OBJECTIVE: This study addresses a critical issue in esophageal cancer study by comparing two different sampling methods, endoscopic brushing and cytosponge, for investigating the esophageal microbiota. Our work highlights the suitability of the cytosponge technique as a minimally invasive sampling method for studying the esophageal microbiota and emphasizes the importance of selecting an appropriate sampling method to characterize the microbial community. Our findings have significant implications for advancing the understanding of the role of the esophageal microbiota in cancer development and will inform future research and clinical approaches in this field.
摘要：
食管癌（EC）是一种多方面的疾病。我们对食管微生物群参与其发病机制和进展的理解是有限的，这是由于缺乏适当的内窥镜采样方法。特此,我们对通过内镜刷洗和细胞海绵获得的配对样本进行了比较分析，旨在评估使用细胞海绵作为研究食管微生物群的微创采样方法的可行性。我们的发现表明，与对照组（非癌性）和EC个体的内窥镜刷洗相比，细胞海绵采样产生了显着优越的社区丰富度和多样性。对β-多样性的分析揭示了两种采样方法在属多样性中不同的微生物群落模式，强调选择适当的采样方法以有效表征食管微生物群的重要性。具体来说,乳球菌和沙雷氏菌在内镜下刷牙采集的样本中显示出较高的丰度，而Alloprevotella和Leptotrichia在通过细胞海绵收集的样品中更富集。优势微生物的这些差异与代谢途径有关，特别是与宿主炎症有关。如丙酮酸和葡萄糖代谢。值得注意的是,对于不同的检测目的，微生物群的系统发育水平显示出不同的解释力。这项研究强调了采样方法选择对与EC发展相关的食管微生物群的获取的重大影响。包括丰富和多样性的考虑。这凸显了选择合适的采样方法对调查食管微生物状况和研究EC相关个体微环境的重要性。
目的：本研究通过比较两种不同的采样方法，解决了食管癌研究中的一个关键问题。内镜下刷牙和细胞海绵，用于研究食道微生物群。我们的工作强调了细胞海绵技术作为研究食管微生物群的微创采样方法的适用性，并强调了选择合适的采样方法来表征微生物群落的重要性。我们的发现对于促进对食管微生物群在癌症发展中的作用的理解具有重要意义，并将为该领域的未来研究和临床方法提供信息。
目的：本研究通过比较两种不同的采样方法，解决了食管癌研究中的一个关键问题。内镜下刷牙和细胞海绵，用于研究食道微生物群。我们的工作强调了细胞海绵技术作为研究食管微生物群的微创采样方法的适用性，并强调了选择合适的采样方法来表征微生物群落的重要性。我们的发现对于促进对食管微生物群在癌症发展中的作用的理解具有重要意义，并将为该领域的未来研究和临床方法提供信息。
