Abstract：
BACKGROUND: Chlorhexidine gluconate (CHG) (0.05%) has recently been suggested as both a dip for the hydrophilic surface and an irrigation solution in the setting of penile prosthesis (PP) surgery.
OBJECTIVE: The study sought to compare the antimicrobial efficacy of 0.05% CHG with vancomycin and gentamicin (VG) antibiotics as dip and/or irrigation solutions in the setting of a hydrophilic PP surface in vitro.
METHODS: Sterile PPs with a hydrophilic coating were obtained. A series of experiments were performed to evaluate the efficacy of normal saline (NS), 0.05% CHG, or VG as dip and/or irrigation solutions to reduce methicillin-sensitive Staphylococcus aureus adhesion to PP surfaces. The 8-mm discs from PPs were incubated in 105 colony-forming units/mL of methicillin-sensitive S aureus for 48 hours, plated, and counted. Disc-diffusion tests were conducted by suspending 6-mm discs for 2 minutes in NS, 0.05% CHG, or VG, then placing them coated side down onto plates streaked with the following organisms: methicillin-sensitive S aureus, S epidermidis, Enterococcus, and Escherichia coli. After 24 hours of growth, zones of inhibition were measured.
RESULTS: We found average bacterial counts (colony-forming units/mL) and zones of inhibition (mm) following a series of treatment protocols of PP discs.
RESULTS: PP discs dipped in VG reduced bacterial adhesion to the implant surface >0.05% CHG (~5.5 log vs ~1.5 log; P < .01). Discs irrigated with either 0.05% CHG or NS removed all dip solution adsorbed to the hydrophilic surface, allowing bacterial growth. VG irrigation adsorbed to the hydrophilic surface even after 0.05% CHG or NS dips, reducing bacterial adherence (~3 log). Dipping and irrigating discs with VG was most effective in reducing adherent bacteria (~5.5 log) and was the only irrigation that showed antimicrobial activity.
CONCLUSIONS: VG, when used both as a prophylactic dip and as an intraoperative irrigation solution for hydrophilic penile implant surfaces, has improved efficacy to 0.05% CHG and NS.
UNASSIGNED: This is the first study to compare the use of VG, 0.05% CHG, and NS as prophylactic dips and intraoperative irrigations for hydrophilic penile implant surfaces. Limitations include the use of in vitro studies, which serve as a proxy for in vivo practices and may not be entirely accurate nor translatable clinically.
CONCLUSIONS: We demonstrated the superior efficacy of VG as a combined dip and irrigation solution for hydrophilic penile implant surfaces compared with 0.05% CHG.
OBJECTIVE: The study sought to compare the antimicrobial efficacy of 0.05% CHG with vancomycin and gentamicin (VG) antibiotics as dip and/or irrigation solutions in the setting of a hydrophilic PP surface in vitro.
METHODS: Sterile PPs with a hydrophilic coating were obtained. A series of experiments were performed to evaluate the efficacy of normal saline (NS), 0.05% CHG, or VG as dip and/or irrigation solutions to reduce methicillin-sensitive Staphylococcus aureus adhesion to PP surfaces. The 8-mm discs from PPs were incubated in 105 colony-forming units/mL of methicillin-sensitive S aureus for 48 hours, plated, and counted. Disc-diffusion tests were conducted by suspending 6-mm discs for 2 minutes in NS, 0.05% CHG, or VG, then placing them coated side down onto plates streaked with the following organisms: methicillin-sensitive S aureus, S epidermidis, Enterococcus, and Escherichia coli. After 24 hours of growth, zones of inhibition were measured.
RESULTS: We found average bacterial counts (colony-forming units/mL) and zones of inhibition (mm) following a series of treatment protocols of PP discs.
RESULTS: PP discs dipped in VG reduced bacterial adhesion to the implant surface >0.05% CHG (~5.5 log vs ~1.5 log; P < .01). Discs irrigated with either 0.05% CHG or NS removed all dip solution adsorbed to the hydrophilic surface, allowing bacterial growth. VG irrigation adsorbed to the hydrophilic surface even after 0.05% CHG or NS dips, reducing bacterial adherence (~3 log). Dipping and irrigating discs with VG was most effective in reducing adherent bacteria (~5.5 log) and was the only irrigation that showed antimicrobial activity.
CONCLUSIONS: VG, when used both as a prophylactic dip and as an intraoperative irrigation solution for hydrophilic penile implant surfaces, has improved efficacy to 0.05% CHG and NS.
UNASSIGNED: This is the first study to compare the use of VG, 0.05% CHG, and NS as prophylactic dips and intraoperative irrigations for hydrophilic penile implant surfaces. Limitations include the use of in vitro studies, which serve as a proxy for in vivo practices and may not be entirely accurate nor translatable clinically.
CONCLUSIONS: We demonstrated the superior efficacy of VG as a combined dip and irrigation solution for hydrophilic penile implant surfaces compared with 0.05% CHG.
摘要：
背景：葡萄糖酸氯己定（CHG）（0.05％）最近被认为是亲水性表面的浸渍和阴茎假体（PP）手术中的冲洗溶液。
目的：本研究试图比较0.05%CHG与万古霉素和庆大霉素(VG)抗生素作为浸渍和/或冲洗溶液在体外亲水性PP表面环境中的抗菌效果。
方法:获得具有亲水涂层的无菌PP。进行了一系列实验以评估生理盐水（NS）的功效，0.05%CHG,或VG作为浸渍和/或冲洗溶液，以减少甲氧西林敏感的金黄色葡萄球菌对PP表面的粘附。将来自PPs的8毫米圆盘在105个菌落形成单位/mL的甲氧西林敏感金黄色葡萄球菌中孵育48小时，电镀,和计数。通过在NS中悬挂6-mm圆盘2分钟进行圆盘扩散测试，0.05%CHG,或VG，然后将它们的涂层侧面朝下放置在标有以下生物的平板上：甲氧西林敏感的金黄色葡萄球菌，表皮葡萄球菌，肠球菌,和大肠杆菌。经过24小时的生长，测量抑制区。
结果:我们发现了一系列PP圆盘处理方案后的平均细菌计数(菌落形成单位/mL)和抑制区(mm)。
结果：浸在VG中的PP圆盘减少了细菌对植入物表面的粘附>0.05％CHG（〜5.5logvs〜1.5log；P<0.01）。用0.05%CHG或NS冲洗的圆盘除去吸附在亲水表面的所有浸液,允许细菌生长。即使在0.05%CHG或NS浸渍后,VG冲洗也吸附在亲水表面上,减少细菌粘附(~3log)。用VG浸渍和灌溉圆盘在减少粘附细菌方面最有效（〜5.5log），并且是唯一显示抗菌活性的灌溉。
结论：VG，当用作亲水性阴茎植入物表面的预防性浸渍和术中冲洗溶液时，具有对0.05%CHG和NS的改善功效。
■这是第一个比较使用VG的研究，0.05%CHG,和NS作为亲水性阴茎植入物表面的预防性浸渍和术中冲洗。局限性包括使用体外研究，作为体内实践的代理，可能不完全准确，也不能在临床上翻译。
结论：我们证明了与0.05%CHG相比，VG作为亲水性阴茎植入物表面的联合浸渍和冲洗溶液具有更好的疗效。
目的：本研究试图比较0.05%CHG与万古霉素和庆大霉素(VG)抗生素作为浸渍和/或冲洗溶液在体外亲水性PP表面环境中的抗菌效果。
方法:获得具有亲水涂层的无菌PP。进行了一系列实验以评估生理盐水（NS）的功效，0.05%CHG,或VG作为浸渍和/或冲洗溶液，以减少甲氧西林敏感的金黄色葡萄球菌对PP表面的粘附。将来自PPs的8毫米圆盘在105个菌落形成单位/mL的甲氧西林敏感金黄色葡萄球菌中孵育48小时，电镀,和计数。通过在NS中悬挂6-mm圆盘2分钟进行圆盘扩散测试，0.05%CHG,或VG，然后将它们的涂层侧面朝下放置在标有以下生物的平板上：甲氧西林敏感的金黄色葡萄球菌，表皮葡萄球菌，肠球菌,和大肠杆菌。经过24小时的生长，测量抑制区。
结果:我们发现了一系列PP圆盘处理方案后的平均细菌计数(菌落形成单位/mL)和抑制区(mm)。
结果：浸在VG中的PP圆盘减少了细菌对植入物表面的粘附>0.05％CHG（〜5.5logvs〜1.5log；P<0.01）。用0.05%CHG或NS冲洗的圆盘除去吸附在亲水表面的所有浸液,允许细菌生长。即使在0.05%CHG或NS浸渍后,VG冲洗也吸附在亲水表面上,减少细菌粘附(~3log)。用VG浸渍和灌溉圆盘在减少粘附细菌方面最有效（〜5.5log），并且是唯一显示抗菌活性的灌溉。
结论：VG，当用作亲水性阴茎植入物表面的预防性浸渍和术中冲洗溶液时，具有对0.05%CHG和NS的改善功效。
■这是第一个比较使用VG的研究，0.05%CHG,和NS作为亲水性阴茎植入物表面的预防性浸渍和术中冲洗。局限性包括使用体外研究，作为体内实践的代理，可能不完全准确，也不能在临床上翻译。
结论：我们证明了与0.05%CHG相比，VG作为亲水性阴茎植入物表面的联合浸渍和冲洗溶液具有更好的疗效。
