PDF(Pubmed)
Abstract：
UNASSIGNED: Microwave ablation (MWA) is a minimally invasive technique for treating lung cancer. It can induce immune response; however, its effect on the immune microenvironment in tumor-draining lymph nodes (TdLN) is not well understood. This study aims to identify changes in the immune microenvironment in TdLN following MWA in a Lewis lung cancer (LLC) mouse model.
UNASSIGNED: LLC mouse model was established and followed by MWA. TdLN were collected at various time points, including pre-MWA and days 1, 2, 4, and 8 post-MWA. Flow cytometry was used to determine the frequencies of CD4+ T cells, CD8+ T cells, regulatory T (Treg) cells, natural killer (NK) cells, dendritic cells (DCs) and other immune cells in the TdLN. Certain cytokines were also detected.
UNASSIGNED: Compared with pre-MWA, the frequency of CD4+ T cells significantly increased from day 1 to day 8 post-MWA. The frequency of CD8+ T cells decreased significantly on days 2 and 4, but no significant changes occurred on days 1 and 8. Significant decreases in the frequencies of Treg cells and Klrg1+ Treg cells were observed from day 1 to day 4. On days 4 and 8, there was a significant increase in the frequency of NK cells. The frequency of resident cDC2 significantly increased on day 4, whereas CD11b+ migratory cDCs increased on day 1. Additionally, on day 4, a notable rise was observed in the frequency of NK cells secreting IFN-γ, while on day 8, there was a significant increase in the frequency of CD8+ T cells secreting both IFN-γ and TNF-α.
UNASSIGNED: MWA of lung cancer can alter the immune microenvironment in the TdLN, triggering immune responses. These changes are particularly evident and intricate within the initial 4 days post-MWA. Treatment combined with MWA within a certain period may significantly enhance anti-tumor immunity.
UNASSIGNED: LLC mouse model was established and followed by MWA. TdLN were collected at various time points, including pre-MWA and days 1, 2, 4, and 8 post-MWA. Flow cytometry was used to determine the frequencies of CD4+ T cells, CD8+ T cells, regulatory T (Treg) cells, natural killer (NK) cells, dendritic cells (DCs) and other immune cells in the TdLN. Certain cytokines were also detected.
UNASSIGNED: Compared with pre-MWA, the frequency of CD4+ T cells significantly increased from day 1 to day 8 post-MWA. The frequency of CD8+ T cells decreased significantly on days 2 and 4, but no significant changes occurred on days 1 and 8. Significant decreases in the frequencies of Treg cells and Klrg1+ Treg cells were observed from day 1 to day 4. On days 4 and 8, there was a significant increase in the frequency of NK cells. The frequency of resident cDC2 significantly increased on day 4, whereas CD11b+ migratory cDCs increased on day 1. Additionally, on day 4, a notable rise was observed in the frequency of NK cells secreting IFN-γ, while on day 8, there was a significant increase in the frequency of CD8+ T cells secreting both IFN-γ and TNF-α.
UNASSIGNED: MWA of lung cancer can alter the immune microenvironment in the TdLN, triggering immune responses. These changes are particularly evident and intricate within the initial 4 days post-MWA. Treatment combined with MWA within a certain period may significantly enhance anti-tumor immunity.
摘要：
■微波消融（MWA）是一种治疗肺癌的微创技术。它可以诱导免疫反应；然而，其对肿瘤引流淋巴结（TdLN）中免疫微环境的影响尚不清楚。这项研究旨在确定Lewis肺癌（LLC）小鼠模型中MWA后TdLN中免疫微环境的变化。建立
■LLC小鼠模型，然后进行MWA。在各个时间点收集TdLN,包括MWA之前和MWA之后的第1、2、4和8天。流式细胞术用于确定CD4+T细胞的频率，CD8+T细胞，调节性T(Treg)细胞,自然杀伤（NK）细胞，TdLN中的树突状细胞(DC)和其他免疫细胞。还检测到某些细胞因子。
■与MWA之前相比，从MWA后第1天到第8天，CD4+T细胞的频率显着增加。CD8+T细胞的频率在第2天和第4天显著降低,但在第1天和第8天未发生显著改变。从第1天至第4天观察到Treg细胞和Klrg1+Treg细胞的频率显著降低。在第4天和第8天,NK细胞的频率显著增加。在第4天，常驻cDC2的频率显着增加，而CD11b迁移cDCs在第1天增加。此外,在第4天，观察到NK细胞分泌IFN-γ的频率显着上升，而在第8天，分泌IFN-γ和TNF-α的CD8T细胞的频率显着增加。
■肺癌的MWA可以改变TdLN中的免疫微环境，触发免疫反应。这些变化在MWA后的最初4天内特别明显和复杂。在一定时期内联合MWA治疗可显著增强抗肿瘤免疫力。
■LLC小鼠模型，然后进行MWA。在各个时间点收集TdLN,包括MWA之前和MWA之后的第1、2、4和8天。流式细胞术用于确定CD4+T细胞的频率，CD8+T细胞，调节性T(Treg)细胞,自然杀伤（NK）细胞，TdLN中的树突状细胞(DC)和其他免疫细胞。还检测到某些细胞因子。
■与MWA之前相比，从MWA后第1天到第8天，CD4+T细胞的频率显着增加。CD8+T细胞的频率在第2天和第4天显著降低,但在第1天和第8天未发生显著改变。从第1天至第4天观察到Treg细胞和Klrg1+Treg细胞的频率显著降低。在第4天和第8天,NK细胞的频率显著增加。在第4天，常驻cDC2的频率显着增加，而CD11b迁移cDCs在第1天增加。此外,在第4天，观察到NK细胞分泌IFN-γ的频率显着上升，而在第8天，分泌IFN-γ和TNF-α的CD8T细胞的频率显着增加。
■肺癌的MWA可以改变TdLN中的免疫微环境，触发免疫反应。这些变化在MWA后的最初4天内特别明显和复杂。在一定时期内联合MWA治疗可显著增强抗肿瘤免疫力。
