UNASSIGNED: LLC mouse model was established and followed by MWA. TdLN were collected at various time points, including pre-MWA and days 1, 2, 4, and 8 post-MWA. Flow cytometry was used to determine the frequencies of CD4+ T cells, CD8+ T cells, regulatory T (Treg) cells, natural killer (NK) cells, dendritic cells (DCs) and other immune cells in the TdLN. Certain cytokines were also detected.
UNASSIGNED: Compared with pre-MWA, the frequency of CD4+ T cells significantly increased from day 1 to day 8 post-MWA. The frequency of CD8+ T cells decreased significantly on days 2 and 4, but no significant changes occurred on days 1 and 8. Significant decreases in the frequencies of Treg cells and Klrg1+ Treg cells were observed from day 1 to day 4. On days 4 and 8, there was a significant increase in the frequency of NK cells. The frequency of resident cDC2 significantly increased on day 4, whereas CD11b+ migratory cDCs increased on day 1. Additionally, on day 4, a notable rise was observed in the frequency of NK cells secreting IFN-γ, while on day 8, there was a significant increase in the frequency of CD8+ T cells secreting both IFN-γ and TNF-α.
UNASSIGNED: MWA of lung cancer can alter the immune microenvironment in the TdLN, triggering immune responses. These changes are particularly evident and intricate within the initial 4 days post-MWA. Treatment combined with MWA within a certain period may significantly enhance anti-tumor immunity.
■LLC小鼠模型,然后进行MWA。在各个时间点收集TdLN,包括MWA之前和MWA之后的第1、2、4和8天。流式细胞术用于确定CD4+T细胞的频率,CD8+T细胞,调节性T(Treg)细胞,自然杀伤(NK)细胞,TdLN中的树突状细胞(DC)和其他免疫细胞。还检测到某些细胞因子。
■与MWA之前相比,从MWA后第1天到第8天,CD4+T细胞的频率显着增加。CD8+T细胞的频率在第2天和第4天显著降低,但在第1天和第8天未发生显著改变。从第1天至第4天观察到Treg细胞和Klrg1+Treg细胞的频率显著降低。在第4天和第8天,NK细胞的频率显著增加。在第4天,常驻cDC2的频率显着增加,而CD11b迁移cDCs在第1天增加。此外,在第4天,观察到NK细胞分泌IFN-γ的频率显着上升,而在第8天,分泌IFN-γ和TNF-α的CD8T细胞的频率显着增加。
■肺癌的MWA可以改变TdLN中的免疫微环境,触发免疫反应。这些变化在MWA后的最初4天内特别明显和复杂。在一定时期内联合MWA治疗可显著增强抗肿瘤免疫力。