PDF(Pubmed)
Abstract：
UNASSIGNED: Colorectal cancer (CRC) is a significant health issue, with notable incidence rates in Norway. The immune response plays a dual role in CRC, offering both protective effects and promoting tumor growth. This research aims to provide a detailed screening of immune-related genes and identify specific genes in CRC and adenomatous polyps within the Norwegian population, potentially serving as detection biomarkers.
UNASSIGNED: The study involved 69 patients (228 biopsies) undergoing colonoscopy, divided into CRC, adenomatous polyps, and control groups. We examined the expression of 579 immune genes through nCounter analysis emphasizing differential expression in tumor versus adjacent non-tumorous tissue and performed quantitative reverse transcription polymerase chain reaction (RT-qPCR) across patient categories.
UNASSIGNED: Key findings include the elevated expression of CXCL1, CXCL2, IL1B, IL6, CXCL8 (IL8), PTGS2, and SPP1 in CRC tissues. Additionally, CXCL1, CXCL2, IL6, CXCL8, and PTGS2 showed significant expression changes in adenomatous polyps, suggesting their early involvement in carcinogenesis.
UNASSIGNED: This study uncovers a distinctive immunological signature in colorectal neoplasia among Norwegians, highlighting CXCL1, CXCL2, IL1B, IL6, CXCL8, PTGS2, and SPP1 as potential CRC biomarkers. These findings warrant further research to confirm their role and explore their utility in non-invasive screening strategies.
UNASSIGNED: The study involved 69 patients (228 biopsies) undergoing colonoscopy, divided into CRC, adenomatous polyps, and control groups. We examined the expression of 579 immune genes through nCounter analysis emphasizing differential expression in tumor versus adjacent non-tumorous tissue and performed quantitative reverse transcription polymerase chain reaction (RT-qPCR) across patient categories.
UNASSIGNED: Key findings include the elevated expression of CXCL1, CXCL2, IL1B, IL6, CXCL8 (IL8), PTGS2, and SPP1 in CRC tissues. Additionally, CXCL1, CXCL2, IL6, CXCL8, and PTGS2 showed significant expression changes in adenomatous polyps, suggesting their early involvement in carcinogenesis.
UNASSIGNED: This study uncovers a distinctive immunological signature in colorectal neoplasia among Norwegians, highlighting CXCL1, CXCL2, IL1B, IL6, CXCL8, PTGS2, and SPP1 as potential CRC biomarkers. These findings warrant further research to confirm their role and explore their utility in non-invasive screening strategies.
摘要：
■结直肠癌（CRC）是一个重要的健康问题，挪威发病率显著。免疫应答在CRC中起着双重作用，提供保护作用和促进肿瘤生长。这项研究旨在提供免疫相关基因的详细筛选，并确定挪威人群中CRC和腺瘤性息肉的特定基因。可能作为检测生物标志物。
■该研究涉及69例接受结肠镜检查的患者（228例活检），分为CRC，腺瘤性息肉,和对照组。我们通过nCounter分析检查了579个免疫基因的表达，强调了肿瘤与相邻非肿瘤组织中的差异表达，并在患者类别中进行了定量逆转录聚合酶链反应（RT-qPCR）。
■主要发现包括CXCL1，CXCL2，IL1B的表达升高，IL6、CXCL8(IL8)、CRC组织中的PTGS2和SPP1。此外,CXCL1、CXCL2、IL6、CXCL8和PTGS2在腺瘤性息肉中表达显著改变,提示它们早期参与癌变.
■这项研究揭示了挪威人结直肠肿瘤的独特免疫学特征，突出显示CXCL1、CXCL2、IL1B、IL6、CXCL8、PTGS2和SPP1是潜在的CRC生物标志物。这些发现值得进一步研究，以证实其作用，并探索其在非侵入性筛查策略中的效用。
■该研究涉及69例接受结肠镜检查的患者（228例活检），分为CRC，腺瘤性息肉,和对照组。我们通过nCounter分析检查了579个免疫基因的表达，强调了肿瘤与相邻非肿瘤组织中的差异表达，并在患者类别中进行了定量逆转录聚合酶链反应（RT-qPCR）。
■主要发现包括CXCL1，CXCL2，IL1B的表达升高，IL6、CXCL8(IL8)、CRC组织中的PTGS2和SPP1。此外,CXCL1、CXCL2、IL6、CXCL8和PTGS2在腺瘤性息肉中表达显著改变,提示它们早期参与癌变.
■这项研究揭示了挪威人结直肠肿瘤的独特免疫学特征，突出显示CXCL1、CXCL2、IL1B、IL6、CXCL8、PTGS2和SPP1是潜在的CRC生物标志物。这些发现值得进一步研究，以证实其作用，并探索其在非侵入性筛查策略中的效用。
