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Abstract：
Pigs (Sus scrofa) are widely acknowledged as an important large mammalian animal model due to their similarity to human physiology, genetics, and immunology. Leveraging the full potential of this model presents significant opportunities for major advancements in the fields of comparative biology, disease modeling, and regenerative medicine. Thus, the derivation of pluripotent stem cells from this species can offer new tools for disease modeling and serve as a stepping stone to test future autologous or allogeneic cell-based therapies. Over the past few decades, great progress has been made in establishing porcine pluripotent stem cells (pPSCs), including embryonic stem cells (pESCs) derived from pre- and peri-implantation embryos, and porcine induced pluripotent stem cells (piPSCs) using a variety of cellular reprogramming strategies. However, the stabilization of pPSCs was not as straightforward as directly applying the culture conditions developed and optimized for murine or primate PSCs. Therefore, it has historically been challenging to establish stable pPSC lines that could pass stringent pluripotency tests. Here, we review recent advances in the establishment of stable porcine PSCs. We focus on the evolving derivation methods that eventually led to the establishment of pESCs and transgene-free piPSCs, as well as current challenges and opportunities in this rapidly advancing field.
摘要：
猪（Susscrofa）由于其与人类生理学的相似性而被广泛认为是一种重要的大型哺乳动物模型。遗传学,和免疫学。充分利用这种模式的全部潜力为比较生物学领域的重大进步提供了重要的机会，疾病建模,和再生医学。因此，从该物种中衍生出多能干细胞可以为疾病建模提供新的工具,并可作为测试未来自体或同种异体细胞疗法的垫脚石.在过去的几十年里，猪多能干细胞(pPSC)的建立取得了很大进展,包括胚胎干细胞（pESCs）来源于植入前和植入期胚胎，和猪诱导多能干细胞(piPSCs)使用多种细胞重编程策略。然而,pPSC的稳定化不像直接应用针对鼠或灵长类动物PSC开发和优化的培养条件那样简单。因此,建立能够通过严格的多能性试验的稳定的pPSC细胞系历来是一项挑战.这里,我们综述了建立稳定的猪PSC的最新进展。我们专注于不断发展的衍生方法，最终导致pESCs和无转基因piPSCs的建立，以及当前这一快速发展领域的挑战和机遇。
