UNASSIGNED: The aim is to develop the natural compound brazilin in the form of a topical cream as an anti-inflammatory agent to reduce NF-κB expression through Imunohistochemistry (IHC) methods, and the expression of pro-inflammatory genes IL-1β, IL-6, p38, and TNF-α.
UNASSIGNED: Male Sprague-Dawley rats were induced with gingivitis using P. gingivalis bacteria. The observed groups included rats treated with a single application of brazilin cream and rats treated with two applications of brazilin cream. The treatment was administered for 15 days. On days 3, 6, 9, 12, and 15, anatomical wound observations and wound histology using hematoxylin-eosin and Masson\'s Trichrome staining were performed. NF-κB protein expression was analyzed using the IHC method. Gingival inflammation gene expression of NF-κB, IL-1β, IL-6, p38, and TNF-α was measured using q-RTPCR.
UNASSIGNED: Single and double applications of brazilin cream increased angiogenesis and decreased NF-κB protein expression, in addition to the IL-1β, IL-6, p38, and TNF-α gene expressions.
UNASSIGNED: In a rat gingivitis model, Brazilin cream may function as an anti-inflammatory agent in the gingival tissue.
■目的是通过免疫组织化学(IHC)方法开发局部乳膏形式的天然化合物brazilin作为抗炎剂,以减少NF-κB的表达,和促炎基因IL-1β的表达,IL-6、p38和TNF-α。
■用牙龈卟啉单胞菌诱导雄性Sprague-Dawley大鼠牙龈炎。观察组包括用单次施用巴西林膏治疗的大鼠和用两次施用巴西林膏治疗的大鼠。治疗施用15天。在第3、6、9、12和15天,使用苏木精-伊红和Masson三色染色进行解剖伤口观察和伤口组织学。使用IHC方法分析NF-κB蛋白表达。牙龈炎症基因NF-κB的表达,IL-1β,使用q-RTPCR测量IL-6、p38和TNF-α。
■单双应用brazilin乳膏可增加血管生成并降低NF-κB蛋白表达,除了IL-1β,IL-6、p38和TNF-α基因表达。
■在大鼠牙龈炎模型中,Brazilin乳膏可以在牙龈组织中用作抗炎剂。