PDF(Pubmed)
Abstract：
UNASSIGNED: Gingivitis is an inflammation of the gums that is the initial cause of the development of periodontal disease by the activity of Nuclear Factor-kappa B (NF-κB), Interleukin-1β (IL-1β), Interleukin-6 (IL-6), p38, and Tumor Necrosis Factor-α (TNF-α). Unaddressed chronic inflammation can lead to persistent disturbances in other parts of the body. Brazilin is a naturally occurring plant chemical that may have antibacterial and anti-inflammatory effects. Treatment based on the natural plant compound, brazilin, is developed in the form of a topical cream for easy application.
UNASSIGNED: The aim is to develop the natural compound brazilin in the form of a topical cream as an anti-inflammatory agent to reduce NF-κB expression through Imunohistochemistry (IHC) methods, and the expression of pro-inflammatory genes IL-1β, IL-6, p38, and TNF-α.
UNASSIGNED: Male Sprague-Dawley rats were induced with gingivitis using P. gingivalis bacteria. The observed groups included rats treated with a single application of brazilin cream and rats treated with two applications of brazilin cream. The treatment was administered for 15 days. On days 3, 6, 9, 12, and 15, anatomical wound observations and wound histology using hematoxylin-eosin and Masson\'s Trichrome staining were performed. NF-κB protein expression was analyzed using the IHC method. Gingival inflammation gene expression of NF-κB, IL-1β, IL-6, p38, and TNF-α was measured using q-RTPCR.
UNASSIGNED: Single and double applications of brazilin cream increased angiogenesis and decreased NF-κB protein expression, in addition to the IL-1β, IL-6, p38, and TNF-α gene expressions.
UNASSIGNED: In a rat gingivitis model, Brazilin cream may function as an anti-inflammatory agent in the gingival tissue.
UNASSIGNED: The aim is to develop the natural compound brazilin in the form of a topical cream as an anti-inflammatory agent to reduce NF-κB expression through Imunohistochemistry (IHC) methods, and the expression of pro-inflammatory genes IL-1β, IL-6, p38, and TNF-α.
UNASSIGNED: Male Sprague-Dawley rats were induced with gingivitis using P. gingivalis bacteria. The observed groups included rats treated with a single application of brazilin cream and rats treated with two applications of brazilin cream. The treatment was administered for 15 days. On days 3, 6, 9, 12, and 15, anatomical wound observations and wound histology using hematoxylin-eosin and Masson\'s Trichrome staining were performed. NF-κB protein expression was analyzed using the IHC method. Gingival inflammation gene expression of NF-κB, IL-1β, IL-6, p38, and TNF-α was measured using q-RTPCR.
UNASSIGNED: Single and double applications of brazilin cream increased angiogenesis and decreased NF-κB protein expression, in addition to the IL-1β, IL-6, p38, and TNF-α gene expressions.
UNASSIGNED: In a rat gingivitis model, Brazilin cream may function as an anti-inflammatory agent in the gingival tissue.
摘要：
■牙龈炎是牙龈的炎症，是通过核因子-κB（NF-κB）的活性引起牙周病发展的最初原因，白细胞介素-1β(IL-1β),白细胞介素-6(IL-6),p38和肿瘤坏死因子-α（TNF-α）。未解决的慢性炎症可导致身体其他部位的持续紊乱。巴西林是一种天然存在的植物化学物质，可能具有抗菌和抗炎作用。基于天然植物化合物的处理，巴西林,以易于应用的局部乳膏的形式开发。
■目的是通过免疫组织化学（IHC）方法开发局部乳膏形式的天然化合物brazilin作为抗炎剂，以减少NF-κB的表达，和促炎基因IL-1β的表达，IL-6、p38和TNF-α。
■用牙龈卟啉单胞菌诱导雄性Sprague-Dawley大鼠牙龈炎。观察组包括用单次施用巴西林膏治疗的大鼠和用两次施用巴西林膏治疗的大鼠。治疗施用15天。在第3、6、9、12和15天，使用苏木精-伊红和Masson三色染色进行解剖伤口观察和伤口组织学。使用IHC方法分析NF-κB蛋白表达。牙龈炎症基因NF-κB的表达,IL-1β,使用q-RTPCR测量IL-6、p38和TNF-α。
■单双应用brazilin乳膏可增加血管生成并降低NF-κB蛋白表达，除了IL-1β，IL-6、p38和TNF-α基因表达。
■在大鼠牙龈炎模型中，Brazilin乳膏可以在牙龈组织中用作抗炎剂。
■目的是通过免疫组织化学（IHC）方法开发局部乳膏形式的天然化合物brazilin作为抗炎剂，以减少NF-κB的表达，和促炎基因IL-1β的表达，IL-6、p38和TNF-α。
■用牙龈卟啉单胞菌诱导雄性Sprague-Dawley大鼠牙龈炎。观察组包括用单次施用巴西林膏治疗的大鼠和用两次施用巴西林膏治疗的大鼠。治疗施用15天。在第3、6、9、12和15天，使用苏木精-伊红和Masson三色染色进行解剖伤口观察和伤口组织学。使用IHC方法分析NF-κB蛋白表达。牙龈炎症基因NF-κB的表达,IL-1β,使用q-RTPCR测量IL-6、p38和TNF-α。
■单双应用brazilin乳膏可增加血管生成并降低NF-κB蛋白表达，除了IL-1β，IL-6、p38和TNF-α基因表达。
■在大鼠牙龈炎模型中，Brazilin乳膏可以在牙龈组织中用作抗炎剂。
