PDF(Pubmed)
Abstract：
UNASSIGNED: Epilepsy is a common and heterogenous neurological disorder characterized by recurrent spontaneous seizures. Animal models like rats play a crucial role in finding of mechanism of epilepsy in different brain regions. i.e., cerebral cortex, cerebellum, hippocampus, and pons medulla. Glutamate is an important excitatory neurotransmitter in the central nervous system and also glutamate plays a vital role in neuronal development and memory. The process of neuronal death evolved by glutamate receptor activation, has been hypothesized in both acute and chronic degenerative disorders including epilepsy. Considering the multifactorial neurochemical and neurophysiological malfunctions consequent to epileptic seizures, a few antiepileptic drugs are designed, to mitigate the debilitating aspects of epilepsy.
UNASSIGNED: Rat model, pentylenetetrazole (PTZ), an anticonvulsant drug, was selected for the present study. Induction of epilepsy/convulsions was induced by an intraperitoneal injection of PTZ (60 mg/kg body weight) in saline. Biochemical assays performed through spectrophotometer.
UNASSIGNED: Glutamine and Glutamine synthetase levels were decreased in the epileptic rats brain regions i.e., hippocampus, cerebellum, cerebral cortex, and pons medulla; glutamate dehydrogenase and glutaminase levels were increased in all the regions of epilepsy induced rats. Highest values are recorded in hippocampus when compared to other brain regions.
UNASSIGNED: PTZ suppresses the function of Glutamine and Glutamine synthetase activities in selected brain regions of rat and enhances the activities of the glutaminase and glutamate dehydrogenase when compared to control rats.
UNASSIGNED: Rat model, pentylenetetrazole (PTZ), an anticonvulsant drug, was selected for the present study. Induction of epilepsy/convulsions was induced by an intraperitoneal injection of PTZ (60 mg/kg body weight) in saline. Biochemical assays performed through spectrophotometer.
UNASSIGNED: Glutamine and Glutamine synthetase levels were decreased in the epileptic rats brain regions i.e., hippocampus, cerebellum, cerebral cortex, and pons medulla; glutamate dehydrogenase and glutaminase levels were increased in all the regions of epilepsy induced rats. Highest values are recorded in hippocampus when compared to other brain regions.
UNASSIGNED: PTZ suppresses the function of Glutamine and Glutamine synthetase activities in selected brain regions of rat and enhances the activities of the glutaminase and glutamate dehydrogenase when compared to control rats.
摘要：
癫痫是一种常见的异质性神经系统疾病，其特征是反复发作的自发性癫痫发作。像大鼠这样的动物模型在发现不同脑区癫痫的机制中起着至关重要的作用。即，大脑皮层,小脑,海马体,和延髓桥.谷氨酸是中枢神经系统中重要的兴奋性神经递质，谷氨酸在神经元发育和记忆中起着至关重要的作用。神经元死亡的过程是由谷氨酸受体激活而进化而来的，已经在包括癫痫在内的急性和慢性退行性疾病中被假设。考虑到癫痫发作后的多因素神经化学和神经生理功能障碍，设计了一些抗癫痫药物，减轻癫痫的衰弱方面。
■大鼠模型,戊四唑(PTZ),抗惊厥药物,被选入本研究。通过腹膜内注射含PTZ(60mg/kg体重)的盐水诱导癫痫/惊厥。通过分光光度计进行生化测定。
■癫痫大鼠脑区的谷氨酰胺和谷氨酰胺合成酶水平降低，即海马体,小脑,大脑皮层,和脑桥延髓；谷氨酸脱氢酶和谷氨酰胺酶水平在癫痫诱发大鼠的所有区域均升高。当与其他脑区域相比时,在海马中记录到最高值。
■与对照大鼠相比，PTZ抑制大鼠选定脑区域中谷氨酰胺和谷氨酰胺合成酶活性的功能，并增强谷氨酰胺酶和谷氨酸脱氢酶的活性。
■大鼠模型,戊四唑(PTZ),抗惊厥药物,被选入本研究。通过腹膜内注射含PTZ(60mg/kg体重)的盐水诱导癫痫/惊厥。通过分光光度计进行生化测定。
■癫痫大鼠脑区的谷氨酰胺和谷氨酰胺合成酶水平降低，即海马体,小脑,大脑皮层,和脑桥延髓；谷氨酸脱氢酶和谷氨酰胺酶水平在癫痫诱发大鼠的所有区域均升高。当与其他脑区域相比时,在海马中记录到最高值。
■与对照大鼠相比，PTZ抑制大鼠选定脑区域中谷氨酰胺和谷氨酰胺合成酶活性的功能，并增强谷氨酰胺酶和谷氨酸脱氢酶的活性。
