PDF(Pubmed)
Abstract：
Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc TR4), poses a significant threat to banana production globally, thereby necessitating effective biocontrol methods to manage this devastating disease. This study investigates the potential of Bacillus siamensis strain JSZ06, isolated from smooth vetch, as a biocontrol agent against Foc TR4. To this end, we conducted a series of in vitro and in vivo experiments to evaluate the antifungal activity of strain JSZ06 and its crude extracts. Additionally, genomic analyses were performed to identify antibiotic synthesis genes, while metabolomic profiling was conducted to characterize bioactive compounds. The results demonstrated that strain JSZ06 exhibited strong inhibitory activity against Foc TR4, significantly reducing mycelial growth and spore germination. Moreover, scanning and transmission electron microscopy revealed substantial ultrastructural damage to Foc TR4 mycelia treated with JSZ06 extracts. Genomic analysis identified several antibiotic synthesis genes, and metabolomic profiling revealed numerous antifungal metabolites. Furthermore, in pot trials, the application of JSZ06 fermentation broth significantly enhanced banana plant growth and reduced disease severity, achieving biocontrol efficiencies of 76.71% and 79.25% for leaves and pseudostems, respectively. In conclusion, Bacillus siamensis JSZ06 is a promising biocontrol agent against Fusarium wilt in bananas, with its dual action of direct antifungal activity and plant growth promotion underscoring its potential for integrated disease management strategies.
摘要：
枯萎病,由尖孢镰刀菌引起。古巴热带种族4（FocTR4），对全球香蕉生产构成重大威胁，因此需要有效的生物防治方法来控制这种破坏性疾病。本研究调查了从光滑紫菜中分离的暹罗芽孢杆菌JSZ06菌株的潜力，作为抗FocTR4的生物防治剂。为此,我们进行了一系列的体外和体内实验，以评估菌株JSZ06及其粗提物的抗真菌活性。此外,进行基因组分析以鉴定抗生素合成基因，同时进行代谢组学分析以表征生物活性化合物。结果表明，菌株JSZ06对FocTR4具有较强的抑制活性，显著降低菌丝生长和孢子萌发。此外，扫描和透射电子显微镜显示，用JSZ06提取物处理的FocTR4菌丝体有很大的超微结构损伤。基因组分析确定了几个抗生素合成基因，代谢组学分析揭示了许多抗真菌代谢物。此外，在大麻试验中，JSZ06发酵液的应用显着增强了香蕉植物的生长并降低了病害的严重程度，叶片和假茎的生物防治效率分别为76.71%和79.25%，分别。总之,芽孢杆菌JSZ06是一种很有前途的抗香蕉枯萎病的生物防治剂，具有直接抗真菌活性和促进植物生长的双重作用，突显了其综合疾病管理策略的潜力。
