PDF(Pubmed)
Abstract：
Here we describe a complex enzymatic approach to the efficient transformation of abundant waste chitin, a byproduct of the food industry, into valuable chitooligomers with a degree of polymerization (DP) ranging from 6 to 11. This method involves a three-step process: initial hydrolysis of chitin using engineered variants of a novel fungal chitinase from Talaromyces flavus to generate low-DP chitooligomers, followed by an extension to the desired DP using the high-yielding Y445N variant of β-N-acetylhexosaminidase from Aspergillus oryzae, achieving yields of up to 57%. Subsequently, enzymatic deacetylation of chitooligomers with DP 6 and 7 was accomplished using peptidoglycan deacetylase from Bacillus subtilis BsPdaC. The innovative enzymatic procedure demonstrates a sustainable and feasible route for converting waste chitin into unavailable bioactive chitooligomers potentially applicable as natural pesticides in ecological and sustainable agriculture.
摘要：
在这里，我们描述了一种复杂的酶促方法来有效转化丰富的废甲壳素，食品工业的副产品，转化为聚合度（DP）为6至11的有价值的壳聚糖低聚物。此方法涉及三步过程：使用来自黄花Talaromyces的新型真菌几丁质酶的工程变体对几丁质进行初始水解，以产生低DP的几丁质低聚物，然后使用米曲霉的β-N-乙酰氨基己糖苷酶的高产Y445N变体扩展到所需的DP，实现高达57%的产量。随后，使用来自枯草芽孢杆菌BsPdaC的肽聚糖脱乙酰酶完成了用DP6和7对壳聚糖低聚物的酶促脱乙酰。创新的酶促程序证明了一种可持续且可行的途径，可将废甲壳素转化为不可用的生物活性壳聚糖低聚物，可用作生态和可持续农业中的天然农药。
