METHODS: Eggs of Ae. albopictus were collected in Matola-Rio, a municipality adjacent to Maputo, and reared to adults in the laboratory. Cytochrome c oxidase subunit I (COI) sequences and microsatellite loci were analyzed to estimate origins. The presence of knockdown resistance (kdr) mutations within domain II and III of the VSSC were examined using Sanger sequencing.
RESULTS: The COI network analysis denied the hypothesis that the Ae. albopictus population originated in Madagascar or IWIO; rather both the COI network and microsatellites analyses showed that the population was genetically similar to those in continental Southeast Asia and Hangzhou, China. Sanger sequencing determined the presence of the F1534C knockdown mutation, which is widely distributed among Asian populations, with a high allele frequency (46%).
CONCLUSIONS: These results do not support the hypothesis that the Mozambique Ae. albopictus population originated in Madagascar or IWIO. Instead, they suggest that the origin is continental Southeast Asia or a coastal town in China.
方法:Ae的鸡蛋。白纹伊蚊在马托拉-里约收集,马普托附近的一个自治市,并在实验室饲养成人。分析了细胞色素C氧化酶亚基I(COI)序列和微卫星基因座以估计起源。使用Sanger测序检查VSSC的结构域II和III内的敲低抗性(kdr)突变的存在。
结果:COI网络分析否定了Ae的假设。白纹伊蚊种群起源于马达加斯加或IWIO;相反,COI网络和微卫星分析表明,该种群在遗传上与东南亚大陆和杭州相似,中国。Sanger测序确定了F1534C敲低突变的存在,在亚洲人群中广泛分布,具有高等位基因频率(46%)。
结论:这些结果不支持莫桑比克Ae的假设。白纹伊蚊种群起源于马达加斯加或IWIO。相反,他们认为原产地是东南亚大陆或中国的沿海城镇。