PDF(Pubmed)
Abstract：
BACKGROUND: The ginseng endophyte Paenibacillus polymyxa Pp-7250 (Pp-7250) has multifaceted roles such as preventing ginseng diseases, promoting growth, increasing ginsenoside accumulation, and degrading pesticide residues, however, these effects still have room for improvements. Composite fungicides are an effective means to improve the biocontrol effect of fungicides, but the effect of Pp-7250 in combination with its symbiotic bacteria on ginseng needs to be further investigated, and its mechanism of action has not been elucidated. In this study, a series of experiments was conducted to elucidate the effect of Paenibacillus polymyxa and Bacillus cereus co-bacterial agent on the yield and quality of understory ginseng, and to investigate their mechanism of action.
RESULTS: The results indicated that P. polymyxa and B. cereus co-bacterial agent (PB) treatment improved ginseng yield, ginsenoside accumulation, disease prevention, and pesticide degradation. The mechanism is that PB treatment increased the abundance of beneficial microorganisms, including Rhodanobacter, Pseudolabrys, Gemmatimonas, Bacillus, Paenibacillus, Cortinarius, Russula, Paecilomyces, and Trechispora, and decreased the abundance of pathogenic microorganisms, including Ellin6067, Acidibacter, Fusarium, Tetracladium, Alternaria, and Ilyonectria in ginseng rhizosphere soil. PB co-bacterial agents enhanced the function of microbial metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, biosynthesis of amino acids, carbon fixation pathways in prokaryotes, DNA replication, and terpenoid backbone biosynthesis, and decreased the function of microbial plant pathogens and animal pathogens.
CONCLUSIONS: The combination of P. polymyxa and B. cereus may be a potential biocontrol agent to promote the resistance of ginseng to disease and improve the yield, quality, and pesticide degradation.
RESULTS: The results indicated that P. polymyxa and B. cereus co-bacterial agent (PB) treatment improved ginseng yield, ginsenoside accumulation, disease prevention, and pesticide degradation. The mechanism is that PB treatment increased the abundance of beneficial microorganisms, including Rhodanobacter, Pseudolabrys, Gemmatimonas, Bacillus, Paenibacillus, Cortinarius, Russula, Paecilomyces, and Trechispora, and decreased the abundance of pathogenic microorganisms, including Ellin6067, Acidibacter, Fusarium, Tetracladium, Alternaria, and Ilyonectria in ginseng rhizosphere soil. PB co-bacterial agents enhanced the function of microbial metabolic pathways, biosynthesis of secondary metabolites, biosynthesis of antibiotics, biosynthesis of amino acids, carbon fixation pathways in prokaryotes, DNA replication, and terpenoid backbone biosynthesis, and decreased the function of microbial plant pathogens and animal pathogens.
CONCLUSIONS: The combination of P. polymyxa and B. cereus may be a potential biocontrol agent to promote the resistance of ginseng to disease and improve the yield, quality, and pesticide degradation.
摘要：
背景：人参内生菌Pp-7250（Pp-7250）具有多方面的作用，例如预防人参疾病，促进增长,增加人参皂苷的积累，降解农药残留，然而,这些效果仍有改进的空间。复合杀菌剂是提高杀菌剂生物防治效果的有效手段,但是Pp-7250及其共生细菌对人参的影响还需要进一步研究，其作用机制尚未阐明。在这项研究中,进行了一系列实验，以阐明多粘类芽孢杆菌和蜡样芽孢杆菌共细菌剂对林下人参的产量和质量的影响。并研究其作用机制。
结果：结果表明，多粘菌和蜡样芽孢杆菌（PB）处理提高了人参产量，人参皂苷积累，疾病预防,和农药降解。其机制是PB处理增加了有益微生物的丰度，包括罗丹菌,伪装者，Gemmatatimonas,芽孢杆菌,拟芽孢杆菌,Cortinarius,Russula,拟青霉,和Trechispora,减少了病原微生物的丰度，包括Ellin6067酸性杆菌,镰刀菌,四片,Alternaria,人参根际土壤中的Ilyonectria。PB助细菌剂增强了微生物代谢途径的功能，次级代谢产物的生物合成，抗生素的生物合成，氨基酸的生物合成，原核生物的碳固定途径，DNA复制,和萜类骨架生物合成，并降低了微生物植物病原体和动物病原体的功能。
结论：多粘菌和蜡样芽孢杆菌的组合可能是促进人参抗病性和提高产量的潜在生物防治剂，质量,和农药降解。
结果：结果表明，多粘菌和蜡样芽孢杆菌（PB）处理提高了人参产量，人参皂苷积累，疾病预防,和农药降解。其机制是PB处理增加了有益微生物的丰度，包括罗丹菌,伪装者，Gemmatatimonas,芽孢杆菌,拟芽孢杆菌,Cortinarius,Russula,拟青霉,和Trechispora,减少了病原微生物的丰度，包括Ellin6067酸性杆菌,镰刀菌,四片,Alternaria,人参根际土壤中的Ilyonectria。PB助细菌剂增强了微生物代谢途径的功能，次级代谢产物的生物合成，抗生素的生物合成，氨基酸的生物合成，原核生物的碳固定途径，DNA复制,和萜类骨架生物合成，并降低了微生物植物病原体和动物病原体的功能。
结论：多粘菌和蜡样芽孢杆菌的组合可能是促进人参抗病性和提高产量的潜在生物防治剂，质量,和农药降解。
