PDF(Pubmed)
Abstract：
BACKGROUND: Hoylesella marshii can be isolated from human oral cavities affected by dental pulp and periodontal infections, as well as from the dental plaque of healthy individuals, making it a common bacterium within the oral microbiota. However, its role in causing pleural infections in humans is rare.
METHODS: A case of purulent pleural effusion occurred shortly after discharge in an elderly patient who had undergone surgery for gastric cancer. The infection was identified as being caused by an obligate anaerobe through laboratory culture, and was further identified as Hoylesella marshii causing pleural infection through 16 S rRNA gene sequence analysis. Susceptibility testing guided precise treatment with cefoperazone-sulbactam and metronidazole. The patient\'s clinical symptoms improved rapidly, laboratory test indicators gradually returned to normal, and the patient ultimately recovered.
CONCLUSIONS: Hoylesella marshii can cause pleural infections in humans. Clinical microbiology laboratories should pay special attention to the cultivation of obligate anaerobes when routine aerobic cultures do not show bacterial growth but bacteria are visible on smear staining, and when conventional identification methods fail to identify the bacterium, analysis based on the highly conserved 16 S rRNA gene sequence can accurately and specifically identify the bacterium, guiding clinicians in formulating precise anti-infection strategies.
METHODS: A case of purulent pleural effusion occurred shortly after discharge in an elderly patient who had undergone surgery for gastric cancer. The infection was identified as being caused by an obligate anaerobe through laboratory culture, and was further identified as Hoylesella marshii causing pleural infection through 16 S rRNA gene sequence analysis. Susceptibility testing guided precise treatment with cefoperazone-sulbactam and metronidazole. The patient\'s clinical symptoms improved rapidly, laboratory test indicators gradually returned to normal, and the patient ultimately recovered.
CONCLUSIONS: Hoylesella marshii can cause pleural infections in humans. Clinical microbiology laboratories should pay special attention to the cultivation of obligate anaerobes when routine aerobic cultures do not show bacterial growth but bacteria are visible on smear staining, and when conventional identification methods fail to identify the bacterium, analysis based on the highly conserved 16 S rRNA gene sequence can accurately and specifically identify the bacterium, guiding clinicians in formulating precise anti-infection strategies.
摘要：
背景：可以从受牙髓和牙周感染影响的人类口腔中分离出来，以及健康个体的牙菌斑，使其成为口腔微生物群中的常见细菌。然而,它在引起人类胸膜感染中的作用很少见。
方法：1例老年胃癌手术患者出院后不久出现脓性胸腔积液。通过实验室培养确定感染是由专性厌氧菌引起的，并通过16SrRNA基因序列分析进一步鉴定为引起胸膜感染的马氏Hoylesella。药敏试验指导头孢哌酮-舒巴坦和甲硝唑的精确治疗。患者的临床症状迅速改善，实验室检测指标逐渐恢复正常,病人最终康复了.
结论：马氏Hoylesella可引起人类胸膜感染。临床微生物学实验室在常规需氧培养不显示细菌生长但涂片染色可见细菌时，应特别注意专性厌氧菌的培养。当传统的鉴定方法无法鉴定细菌时，基于高度保守的16SrRNA基因序列的分析可以准确、特异地鉴定细菌,指导临床医生制定精确的抗感染策略。
方法：1例老年胃癌手术患者出院后不久出现脓性胸腔积液。通过实验室培养确定感染是由专性厌氧菌引起的，并通过16SrRNA基因序列分析进一步鉴定为引起胸膜感染的马氏Hoylesella。药敏试验指导头孢哌酮-舒巴坦和甲硝唑的精确治疗。患者的临床症状迅速改善，实验室检测指标逐渐恢复正常,病人最终康复了.
结论：马氏Hoylesella可引起人类胸膜感染。临床微生物学实验室在常规需氧培养不显示细菌生长但涂片染色可见细菌时，应特别注意专性厌氧菌的培养。当传统的鉴定方法无法鉴定细菌时，基于高度保守的16SrRNA基因序列的分析可以准确、特异地鉴定细菌,指导临床医生制定精确的抗感染策略。
