Abstract：
With impaired retinal ganglion cell (RGC) function and eventual RGC death, there is a heightened risk of experiencing glaucoma-induced blindness or other optic neuropathies. Poor RGC efficiency leads to limited transmission of visual signals between the retina and the brain by RGC axons. Increased focus on studying lipid messengers found in neurons such as endocannabinoids (eCBs) has importance due to their potential axonal pathway regenerative properties. 2-Arachidonoylglycerol (2-AG), a common eCB, is synthesized from an sn-1 hydrolysis reaction between diacylglycerol (DAG) and diacylglycerol lipase (DAGL). Examination of DAG production allows for future downstream analysis in relation to DAGL functionality. Here, we describe protocol guidelines for extracting RGCs from mouse retinas and subsequent mass spectrometry analysis of the DAG content present within the RGCs.
摘要：
视网膜神经节细胞（RGC）功能受损并最终导致RGC死亡，患青光眼致盲或其他视神经病变的风险增加.差的RGC效率导致视网膜和大脑之间通过RGC轴突的视觉信号的有限传输。由于其潜在的轴突途径再生特性，因此越来越关注研究在神经元中发现的脂质信使，例如内源性大麻素（eCB）具有重要意义。2-花生四酰基甘油（2-AG），常见的eCB，由二酰甘油(DAG)和二酰甘油脂肪酶(DAGL)之间的sn-1水解反应合成。对DAG生产的检查允许未来与DAGL功能相关的下游分析。这里,我们描述了从小鼠视网膜中提取RGCs以及随后对RGCs中存在的DAG含量进行质谱分析的方案指南.
